采用聚丙烯酰胺凝胶垂直板电泳法对蓝色鳞鲤(Cyprinus carpio blue var)进行同工酶分析。结果表明:在蓝色鳞鲤的眼睛、肌肉、肝脏、心脏、肾脏5种组织中,14种酶(LDH、ADH、GDH、MDH、G-6-PDH、EST、POD、SDH、FDH、SOD、α-AMY、CAT、COX、ME)的同工酶谱均存在明显的组织特异性。14种酶共记录出33个基因位点,其中α-Amy-2、Cox-2、Est-1、Ldh-1、Mdh-1、Mdh-2和Sod-1为多态位点。蓝色鳞鲤群体的多态位点百分数为21.21%(P0.99),平均预期杂合度和平均实际杂合度分别为0.1079和0.2121,遗传偏离指数(d)值为正。平均有效等位基因数(Ne)为1.24。实验表明,目前蓝色鳞鲤群体的种质资源状况尚好,表现出明显的杂交优势。
The levels of genetic variation at 40 genic loci of 15 isozymic systems in rainbow trout(Oncorhynchus mykiss) were determined among Donaldsons super rainbow trout,rainbow trout from California of USA,rainbow trout from Norway, rainbow trout from Danmark,and Jalo rainbow trout respectively.The genetic parameters would be important in selective breeding programs.As a main research means,the discontinuous vertical plate polyacrylamide gel electrophoresis(PAGE) was used to analyze the biochemical genetics status of 5 populations of rainbow trout.The P of 5 populations of rainbow trout ranged from 43.24% to 47.37%;the He′ of 5 populations of rainbow trout ranged from 0.1917 to 0.2178;the Ho′ ranged from 0.2703 to 0.3291;the D ranged from 0.4100 to 0.5331;the Ne ranged from 1.4865 to 1.5385.The chi-Square test showed all polymorphic loci from the 5 populations of rainbow trout were in Hardy-Weinberg equilibrium.Various genetic indexes showed that resource quality of the 5 populations of rainbow trout resource quality was still good.Phylogenetic tree showed rainbow trout from Norway,rainbow trout from Danmark,rainbow trout from California of USA and Donaldsons super rainbow trout belonged to the same clade,and Jalo rainbow trout belonged to another clade.
