Because of the difficulty to obtain the traffic flow information of lanes at non-detector intersections in most metropolises of the world,based on the relationships between the lanes of signal-controlled intersections,cluster analysis and stepwise regression are integrated to predict the traffic volume of lanes at non-detector isolated controlled intersections.First cluster analysis is used to cluster the lanes of non-detector isolated signal-controlled intersections and the lanes of all signal-controlled intersections with detectors.Then, by the results of cluster analysis,the traffic volume samples are selected randomly and stepwise regression is used to predict the traffic volume of lanes at non-detector isolated signal-controlled intersections.The method is tested by the traffic volume data of lanes of the road network of Nanjing city.The problem of predicting the traffic volume of lanes at non-detector isolated signal-controlled intersections was resolved and can be widely used in urban traffic flow guidance and urban traffic control in cities without enough intersections equipped with detectors.