The situation of intercepted alien pests from imported hides in Hebei during 2013-2014 was systematically analyzed. The results showed that 163 batches of alien pests were intercepted, including 2 batches of quarantine weeds and they both were Italian cocklebur. According to the statistics of original countries, the intercepted hides from 21 countries all had alien pests, in which Australia was the largest number of batches and species of alien pests. The analysis results showed that there was severe risk in the imported hides carrying alien pests, thus to effectively prevent the invasion of alien pests to our country, several quarantine measures should be taken in the future, such as enhancing the quarantine inspection in ports, and promoting the personnel ability of interception and species identification of alien pests.
为了解奶牛皮肤真菌病的病原及其对常见抗真菌药物的敏感性,给临床治疗提供依据,从具有皮肤真菌病症状奶牛病灶处的皮屑样品中,使用沙氏培养基分离病原真菌,通过核糖体r DNA ITS序列对其分析鉴定。使用ATB FUNGUS 3真菌药敏试纸条测定5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑及伏立康唑对所分离病原真菌的最低抑菌浓度(MIC)。结果表明,在沙氏培养基上分离到的10株中心蜡状成堆、边缘"杉树状"的真菌,经ITS序列分析,鉴定为人兽共患性致病真菌——疣状毛癣菌;所分离的疣状毛癣菌对伏立康唑、伊曲康唑均为敏感,对氟康唑均为中介,而对两性霉素B及5氟胞嘧啶均为耐药;ITS序列分析可实现对真菌的快速、准确鉴定;伏立康唑、伊曲康唑对本研究中分离的疣状毛癣菌具有良好的体外抗菌活性。
