With tomato paste as raw material, the optimal extraction process of phy- toene from tomato paste was explored by orthogonal experiment on the basis of single-factor experiments. The results showed that the optimal extraction process for phytoene from tomato paste was as follows： extractant, acetone; solid to liquid ratio, 10：1; extraction time, 40 min; extraction temperature, 45 ℃. The developed extrac- tion process was stable, reasonable and feasible. Under the optimal extraction pro- cess, the extraction rate of phytoene reached 0.044 4%.
Aim To Methods The constituents tures were identified investigate the chemical constituents of MiUettia nitita var. hirsutissima. were isolated and purified by chromatographic techniques, and the strucby spectral evidences. Results Four flavonoids were isolated from the plant, including liquiritigenin （ 1 ）, naringenin （2）, maackiain （3）, and 3R-vestitol （4）. Conclusion Compounds 1 and 2 were obtained from the genus Millettia for the first time ; 3 and 4 were obtained from the plant for the first time.
