目的观察补中益气汤灌胃对右下肺癌肺叶切除术后大鼠外周血T细胞水平的影响。方法 Wistar大鼠80只,制备大鼠右下肺鳞癌模型。取模型制备成功大鼠60只,将其随机分为实验组及对照组各30只,均行右下肺叶切除。自术后次日实验组经口给予补中益气汤灌胃,100 m L/(kg·d);对照组等量生理盐水灌胃。右下肺叶切除术中取各组右下肺血管内不凝血50μL作为术前血;术后第3、7、14天分别处死实验组、对照组10只大鼠,取大鼠眼眶静脉不凝血50μL。用流式细胞仪检测各组大鼠术前及术后第3、7、14天外周血CD3^+、CD4^+、CD8^+T细胞水平,并计算CD4^+/CD8^+。结果与本组术前相比,术后实验组、对照组术后第3天外周血CD3^+、CD4^+T细胞水平及CD4^+/CD8^+均低,CD8^+T细胞水平高(P均<0.05)。实验组术后第7天较术前及对照组术后第7天外周血CD3^+、CD4^+T细胞水平及CD4^+/CD8^+高,CD8^+T细胞水平低(P均<0.05)。与本组术前相比,实验组和对照组术后第14天外周血CD3^+、CD4^+T细胞水平及CD4^+/CD8^+均高,CD8^+T细胞水平均低(P均<0.05),而术后两组相比P均>0.05。实验组术后第3、7、14天外周血CD3^+、CD4^+、CD8^+T细胞水平及CD4^+/CD8^+两两相比P均<0.05。结论补中益气汤灌胃可提高右下肺癌肺叶切除术后大鼠外周血CD3^+、CD4^+T细胞水平及CD4^+/CD8^+,并降低CD8^+T细胞水平。
Objective: To study the multidrug resistance (MDR) mechanism of lung resistance protein (LRP) gene in hepatocellular carcinoma (HCC), and the relations among the expression of the LRP gene and clinicopathologic features, the influence of α-fetoprotein (AFP), and prognosis of patients who received adjuvant chemotherapy after resection of HCC. Methods: The expression of the LRP gene encoding LRP and mRNA LRP was detected in tissues from 54 untreated patients with HCC, adjacent tissues from 24 patients with HCC and archival paraffin-embedded tissues from 12 patients with posthepatitic cirrhosis. The relationship between the LRP gene expression and the change of AFP level was analyzed in the 24 postoperative HCC patients whose AFP was measured after 2 weeks. All of the HCC patients were followed up. Results: The percentage of positive expression of LRP and mRNA LRP in the 3 tissues was 61.1%, 33.3%, 16.7%, and 75.9%, 37.5%, 33.3% respectively. There was significant difference between the untreated HCC tissue and other tissues (P<0.05). No difference existed between the LRP gene expression and clinicopathologic findings, age, sex, and tumor size (P>0.05), but the expression was related to the degree of differentiation of HCC (P<0.05). The effective rate of AFP in the LRP gene positive expression group or in postoperative chemotherapeutic patients was very lower than that in the negative group (P<0.05). Although the mean survival time of postoperative HCC patients in negative LRP gene expression group was longer than that of positive group, there was no difference between them (P<0.05). Conclusion: LRP gene expression is related to MDR of HCC and initiates the intrinsic MDR. Detection of LRP gene expression is of great guiding significance in accessing chemotherapeutic resistance of HCC. As an index to chemotherapy of HCC, detection of LRP expression provides evidence for making individual chemotherapeutic treatment,and reversing MDR in HCC. Although LRP gene expression correlates with the tumor differential degree (P
