目的:硅是影响荔枝裂果的重要因素之一。对抗裂性不同的两个荔枝品种(‘糯米糍’和‘桂味’荔枝)进行不同浓度的硅肥处理,以期了解荔枝果皮硅含量、果皮硬度、果实发育状况与果实裂果间的关系。结果:1) 叶片喷施不同浓度硅肥后,都可增加荔枝果皮的硅含量。2) 荔枝叶片喷施不同浓度硅肥后,浓度为500 ppm和2000 ppm的硅肥可以增加‘桂味’荔枝果皮的最大力值和果皮最大应力值。3) 喷施硅肥的‘糯米糍’荔枝其单果重、果皮重都比对照高,种子重都比对照低。4) 喷施硅肥的‘桂味’和‘糯米糍’荔枝裂果率都比对照低,并达显著水平。Objective: Silicon is one of important factors that affect fruit cracking of litchi. Two litchi varieties (‘Nuo Mici’ and ‘Gui Wei’) of different cracking resistance were sprayed by different concentration silicon fertilizer. The relationship between silicon content in litchi pericarp, hardness of pericarp, fruit development and fruit crack was studied. Result: 1) The silicon content in litchi pericarp was increased after litchi leaves were sprayed by different concentrations of silicon fertilizer. 2) The maximum force and maximum stress value of ‘Gui Wei’ litchi pericarp were increased after ‘Gui Wei’ litchi leaves were sprayed by concentration of 500 ppm and 2000 ppm of silicon fertilizer. 3) The weight of single fruit and pericarp of ‘Nuo Mici’ litchi that was sprayed by silicon fertilizer were higher than that of contrast, the weight of seed were all lower than that of contrast. 4) The fruit cracking rate of ‘Gui Wei’ and ‘Nuo Mici’ litchi that were sprayed by silicon fertilizer were lower than that of contrast, and that achieves the remarkable level.
辣椒素类物质是辣椒属植物特有的一种次生代谢产物,特异地在辣椒果实胎座中合成,且具有明显的发育阶段特异性。辣椒素合成途径相关基因主要在辣椒花后16 d胎座发育至绿熟期转录,然而转录调控的精细分子机制尚不太清楚。用已知参与调控辣椒素生物合成的转录因子为诱饵,采用酵母双杂交技术从辣椒素合成阶段酵母cDNA文库中筛选与其互作的蛋白,有利于明确辣椒素生物合成的转录调控机制。因此,获得辣椒素合成阶段的高质量酵母cDNA文库是关键。本研究采用辣椒‘59’自交系花后16 d(DPA)的RNA作为模板,之后用SMART c DNA/均一化c DNA文库技术(Switching mechanism at 5’end of the RNA transcript)与同源重组的方法将‘59’自交系的胎座cDNA文库转化酵母感受态Y187。通过对收集的文库进行鉴定发现,滴度达到3.63×10~7CFU/m L,插入片段大约250~2000 bp,文库满足酵母双杂交要求。辣椒胎座高质量cDNA文库的构建为后续辣椒素生物合成的转录调控网络研究提供了基础。
