[Objective] The aim was to study the effects of combined of manure and fertilizer on the content of nutrients in purplish soil as well as release dynamics of enzyme activities.[Method] The effects of manure and fertilizer application on the content of AOM,NH+4-N and NO-3-N in soil as well as release dynamics of enzyme activities were studied by means of simulation culturing.[Result] In the treatments of combined application of simulation culture experiment,the content of active organic matters,NH+4-N,NO-3-N and soil enzyme activities changed dynamically,which showed a shape of inverted "S" with two or more peaks.Compared with the treatment of fertilization,their peaks were relatively flat and occurred relatively late,while the treatment of fertilization had only one but the highest peak.The content of active organic matter came to top around 10 d in the order of cow5fertilizer5pig5fertilizer5cow manurepig manurefertilizer Ⅰfertilizer Ⅱ;the activity of urease came to top around 10 d in all treatments and in the order of pig manurecow5fertilizer5cow manurefertilizer Ⅰfertilizer Ⅱpig5fertilizer5.The activity of saccharase was in the order of pig5fertilizer5pig manurecow5fertilizer5cow manureCKfertilizer Ⅱfertilizer Ⅰ.[Conclusion] The study could provide the theoretical basis for reasonable application of manure and fertilizer.