Primers FP/ RP and TaqMan-MGB Probe were designed from sequence of apxIVA gene specific to all serotypes of Actinobacillus pleuropneumoniae. A real-time PCR method was developed for detecting Actinobacillus pleuropneumoniae, which can amplify all strains of APP. The sensitivity is 12fg DNA or 5.1CFU bacteria. This method can be directly used for detection of APP without DNA extraction.
