您的位置: 专家智库 > >

蒋明玉

作品数:3 被引量:12H指数:2
供职机构:复旦大学附属儿科医院中西医结合科更多>>
发文基金:国家自然科学基金更多>>
相关领域:医药卫生更多>>

文献类型

  • 3篇中文期刊文章

领域

  • 3篇医药卫生

主题

  • 2篇激素
  • 1篇氧化氮
  • 1篇一氧化氮
  • 1篇神经内分泌
  • 1篇神经内分泌系...
  • 1篇肾阴
  • 1篇生长激素
  • 1篇受体
  • 1篇受体表达
  • 1篇染毒
  • 1篇染毒大鼠
  • 1篇中药
  • 1篇相火
  • 1篇内分泌
  • 1篇内分泌干扰
  • 1篇内分泌干扰物
  • 1篇内分泌系统
  • 1篇精氨酸
  • 1篇环境内分泌干...
  • 1篇激素受体

机构

  • 3篇复旦大学
  • 1篇首都医科大学...

作者

  • 3篇蔡德培
  • 3篇蒋明玉
  • 1篇孔元原

传媒

  • 1篇中国中西医结...
  • 1篇国际内分泌代...
  • 1篇Neuros...

年份

  • 2篇2011
  • 1篇2010
3 条 记 录,以下是 1-3
排序方式:
一氧化氮在L-精氨酸促生长激素分泌中的作用被引量:1
2011年
氨基酸是促进垂体生长激素(GH)分泌的潜在刺激因子。在所有氨基酸中,左旋精氨酸(L-精氨酸)促GH分泌作用最为明显。口服或静脉滴注精氨酸均可刺激垂体GH的分泌。L-精氨酸促GH的分泌可能涉及多种机制,但一氧化氮(NO)依赖的可溶性鸟苷酸环化酶-环鸟苷酸-蛋白激酶G(sGC-cGMP-PKG)信号转导通路在此过程中起着重要作用。
蒋明玉蔡德培
关键词:精氨酸一氧化氮生长激素神经内分泌系统
滋肾阴泻相火中药对环境内分泌干扰物染毒大鼠生殖器官雌激素受体表达的影响被引量:8
2010年
目的观察滋肾阴泻相火中药对壬基酚及双酚A染毒大鼠子宫、卵巢雌激素受体(ER)基因和蛋白表达的影响,探讨该中药拮抗环境内分泌干扰物(EEDs)拟雌激素活性的作用机制。方法3周龄雌性SD大鼠染毒组单纯喂饲壬基酚或壬基酚与双酚A联合喂饲,治疗组以滋肾阴泻相火中药与染毒物同时喂饲,对照组喂饲溶剂玉米油,给药15天,分别检测子宫湿重及脏器系数、子宫及卵巢ER基因和蛋白表达水平。结果染毒组与对照组比较,子宫湿重及子宫系数增加,ER蛋白表达显著上调;卵巢ERα、ERβ的基因表达及蛋白水平显著上调(P<0.05)。治疗组与染毒组比较,子宫湿重、子宫系数降低,ER的蛋白表达显著下调;卵巢ERα、ERβ的基因表达及蛋白水平显著下调(P<0.05)。结论EEDs对生殖器官有显著的拟雌激素作用,而滋肾阴泻相火中药对EEDs的拟雌激素活性具有显著的拮抗作用。中药的治疗干预可使生殖器官ER基因、蛋白表达显著下调,从而显著降低生殖器官对EEDs的敏感性,这可能是所用中药有效拮抗EEDs拟雌激素活性的主要作用机制之一。
孔元原蒋明玉蔡德培
关键词:环境内分泌干扰物雌激素受体
Oral arginine improves linear growth of long bones and the neuroendocrine mechanism被引量:3
2011年
Objective To investigate the effect of oral administration of arginine on linear growth of long bones in male pubertal rats and the underlying mechanisms, focusing on expression of genes related to the hypothalamus-pituitary growth axis and the nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathway. Methods Rats were randomly divided into control and intervention groups. In the intervention group, arginine was solved in water (0.045 g L-arginine was mixed with 1 mL water) and administered in rats (10 mL/kg) through gastric perfusion once per day, for totally 28 d. Rats in the control group received normal saline treatment. Bone histomorphometry analysis was used to measure growth plate width and mineral apposition rate of the tibia, as well as trabecular bone volume fraction, osteoblast surface and osteo- clast surface of the femur. Serum growth hormone (GH) concentration was determined by radioimmunoassay. Real-time PCR was used to measure the expression of neuronal nitric oxide synthase (nNOS), soluble guanylyl cyclases (sGCal and sGCfll), growth hormone-releasing hormone (Ghrh) and somatostatin (SS) in hypothalamus, as well as Gh in pituitary. Western blot was used to detect the protein levels ofnNOS, sGCα1 and sGCβ1 in hypothalamus. Results After treat- ment with arginine, the growth plate width of tibia and osteoblast surface of femur were increased (P 〈 0.05), and serum GH concentration was elevated (P 〈 0.05). Besides, mRNA and protein levels ofnNOS and sGCα1 (P 〈 0.05), as well as the expression of Gh mRNA (P 〈 0.01), were significantly up-regulated, while the expression of SS mRNA was down- regulated (P 〈 0.05). Conclusion Oral administration ofarginine could improve linear growth of long bones by regulating mRNA expression of SS and Gh and inducing GH secretion, possibly via nNOS-NO-sGC-cGMP signal transduction pathway.
蒋明玉蔡德培
关键词:ARGININE
共1页<1>
聚类工具0