目的初步确定胃癌和消化性溃疡、胃炎幽门螺杆菌(Hp)的差异蛋白质。方法通过从胃镜活检胃黏膜组织中分离培养 Hp,应用裂解液、超声破碎法提取 Hp 菌体蛋白质后经 Bradford法进行菌体蛋白质定量,采用双向电泳方法获得 Hp 菌体蛋白质图像,运用 ImageMaster5.0版图像分析软件找到胃癌与消化性溃疡、胃炎 Hp 的差异蛋白质,应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)和四极杆飞行时间电喷雾串联质谱(Q-TOF)鉴定差异蛋白质。最后,通过WWW.matrixscience.com,从 mascot 数据库检索差异蛋白质。结果选择胃癌、消化性溃疡和胃炎 Hp各3例进行双向电泳,与胃溃疡和胃炎相比,胃癌 Hp 中有4个蛋白质点高表达,质潜鉴定结果为硫氧还蛋白、腺苷酸激酶、单链 DNA 结合蛋白和核糖体蛋白,50 S,L7/L12,其 Mowse 分值分别为94、286、139和132,序列覆盖率分别为77%、33%、33%和28%。结论硫氧还蛋白具有抗氧化和抑制细胞凋亡的功能,可能与 Hp 的致癌作用相关,蛋白质组学研究在 Hp 与胃癌关系的研究领域有广泛的应用前景。
The differentially expressed proteins between normal and tamoxifen-induced autophagic MCF-7 cells were studied by 2-DE combining with mass spectrometric analysis.Totally,there were 668±99 and 550±97 protein spots detected in the 2-DE maps from normal and autophagic MCF-7 cells respectively,among which 5 changed protein spots were identified by the Q-TOF MS/MS and database search.
