目的:观察针刺对卒中后抑郁大鼠脑组织去甲肾上腺素神经递质五羟色胺(5-HT)转运体(5-HTT)、5-HT_(1A)受体(5-HT_(1A) R)、去甲肾上腺素α_2受体(NEα_2R)m RNA的影响。方法:将40只雄性SD大鼠随机分为正常组、模型组、药物组、针刺组,每组各10只。采用大脑中动脉闭塞和慢性不可预见性温和性应激法复合制备卒中后抑郁模型。造模成功后,正常组、模型组不予干预,药物组予以氟西汀(2 mg/kg)灌胃,针刺组选取"百会""风府""神门""太冲"穴,针刺20 min,期间行针1次,每日1次,7 d为一疗程,共3个疗程,疗程之间休息1 d。观察干预后各组大鼠行为学及5-HT、NE变化,采用逆转录聚合酶链式反应(RT-PCR)观察海马、中缝核、蓝斑核5-HTT、5-HT_(1A) R、NEα_2R m RNA变化。结果:治疗后,与正常组比较,模型组Zea Longa评分升高,糖水消耗量、敞箱水平运动、敞箱垂直运动次数降低(均P<0.01);与模型组比较,针刺组、药物组Zea Longa评分降低,糖水消耗量、敞箱水平运动、敞箱垂直运动次数升高(P<0.01,P<0.05);针刺组和药物组比较,大鼠行为学变化无统计学意义(均P>0.05)。治疗后,与正常组比较,模型组脑组织5-HT、NE及海马、中缝核、蓝斑核5-HTT m RNA、5-HT_(1A) R m RNA表达降低(均P<0.01),NEα_2R m RNA升高(P<0.01);与模型组比较,针刺组、药物组脑皮质5-HT、NE含量明显升高(均P<0.01),海马、中缝核、蓝斑核5-HTT、5-HT_(1A) R mRNA表达升高(P<0.01,P<0.05),NEα_2R m RNA降低(均P<0.01);针刺组与药物组比较差异无统计学意义(均P>0.05)。结论:针刺能明显改善卒中后抑郁大鼠行为学变化,其机制可能与调节大鼠脑皮质5-HT、NE及海马、中缝核、蓝斑核5-HTT、5-HT_(1A)R、NEα_2R mRNA表达有关。
Objective To observe the clinical efficacy of treating simple pediatric diarrhea by chiropractic therapy. Methods Forty-two cases of simple diarrhea were treated with chiropractic therapy along the points from Guiwei （龟尾） to Dazhui （大椎 GV 14）, once a day, with a total of three times. The clinical efficacy was observed. Results Thirty-eight cases were cured （90.5%） and 4 cases were improved （9.5%） with the total effective rate of 100%. Conclusion The chiropractic therapy has good efficacy in treating simple pediatric diarrhea and worthy of clinical promotion.
