Abstract AIMS To investigate the correlation between microvessel count and various clinicopathologic factors and prognosis of gastric carcinoma using immunohistochemical staining with anti factor Ⅷ related antigen (F ⅧRAg) antibody. METHODS A total of 128 specimens resected from patients with gastric carcinoma were investigated by immunohistochemical staining with a monoclonal antibody against F ⅧRAg. Correlations between the microvessel count (the mean number of microvessel in the five areas of highest vascular density at 200× magnification) and various clinicopathologic factors and prognosis of 86 cases with complete follow up data were studied.
胃癌存档石腊组织110例测定其细胞 DNA 含量的 FCM,分析 DNA 倍体及其他病理因素与预后的关系。结果:异倍体的检出率为65%;胃癌患者5年生存率异倍体型与二倍体分别为22.2%和55.3%(P<0.01)。肿瘤浸润深度、生长方式、分化程度、淋巴结转移及 TNM 分期对预后有显著影响,Cox 模型多因素分析结果提示,DNA 倍体是重要影响胃癌预后的独立因素。
AIMS To investigate the clinical significance of vas- cular endothelial growth factor (VEGF) expression in gastric carcinoma. METHODS The expression of VEGF in 128 gastric carcinomas was investigated by immunohistochemical staining with a polyclonal antibody against VEGF. Cor- relations between the expression of VEGF and various clinicopathologic factors and prognosis were studied. RESULTS The VEGF-rich expression rate was 64.1% in gastric carcinoma. VEGF-rich expression rate of patients with stage Ⅲ and stage Ⅳ disease was greater than that of patients with stage f disease (P <0.05). Significant differences of expression rate ex- isted with respect to growth pattern,serosal invasion and lymph node metastasis. The VEGF-rich rate was much higher in tumors with expanding growth pattern (71.8%) or serosal invasion (73.5%) than in those with infiltrative growth pattern (52.0%) or non-serosal invasion (53.3%) (P<0.025,respectively),and it was also significantly higher in patients with lymph node metastases (75.0%) than in those without such metastases (50.0%) (P<0.05). In addition,postop- erative survey of 86 patients who had been followed up for at least 5 years demonstrated that the 5-year sur- vival rate of patients with VEGF-rich tumors was signifi- cantly lower than that of patients with VEGF-poor tu- mors (P<0.05). CONCLUSIONS The expression of VEGF may be as- sociated with the invasion and metastasis and may also be a useful prognostic indicator of gastric carcinoma.
