[Objective] The artificial breeding technology of Cuban fly （Lixophaga diatraeae） and its parasitic ability on Chilo infuscatellus was studied.[Method] Through artificial inoculation experiment,the effects of different inoculation proportions between maggot and Galleria mellonella larvae on the growth and development of Cuban fly were studied.Through indoor low temperature storage tests,effects of different temperatures on the emergence rate of Cuban fly were studied;and the effects of different ecological conditions （humidity,sunshine） on the emergence rate of Cuban fly and its parasitic ability on Chilo infuscatellus were studied through indoor and field comparison experiment.[Result] The optimum inoculation proportions between maggot and G.mellonella larvae were 3：1 and 4：1,pupa production amounts were 279 and 327,respectively;short storage under the temperature of 10℃ had small effect on the emergence rate of Cuban fly,while the storage time should not exceed 15 d.Indoor and field parasitic test showed that Cuban fly had strong parasitic ability on C.infuscatellus,the parasitic rate reached 33.3%-50.0%;the parasitic rates during elongation stage and maturation stage were 47.8% and 41.7%,respectively,while they almost could not parasite during seedling stage.[Conclusion] The results provided scientific basis for artificial breeding of Cuban fly in large scale and its field release technology.
