The pollen of two tomato varieties, Ryau961721 and Ryau9327D, was adopted in our research. The two tomato varieties were bred by College of Land- scape and Horticulture, Yunnan Agricultural University. The collected pollen was stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circumstances. Then it was inoculated to the medium and cultured at 28 ℃ in thermostat incubator. The pollen viability was determined by electron microscope. The results showed that compared to that of pollen stored in control （25 ℃） circumstance, the viability of pollen stored in low-temperature （4 ℃） and ultra-low-temperature （-196 ℃） circum- stances for 1 -3 d did not change significantly. In addition, pollen viability trended to decrease with the increase of freeze-thaw cycle and storage time. The pollen lost basically the viability by the 7th d in the storage.
[Objective] This study aimed to provide a basis for enriching the Punica granatum L. germplasm resources through investigating Punica granatum L. vari- eties in Binchuan. [Method] In this study, biological traits of Punica granatum L. vari- eties in Binchuan （including two local varieties-＇Binchuan Red Coat＇ and ＇Binchuan Green Coat＇, ＇Cyan Coat and Soft Seeds＇ and ＇Little Red Coat＇ introduced from Huili, Sichuan, ＇Acid Pomegranate＇ introduced from Shandong Province and ＂Ma Pomegranate＂ from Fengqiu, Henan） were investigated and their major fruit qualities were also analyzed. [Result] ＂Cyan Coat and Soft Seeds＇ and ＂Ma Pomegranate＇ grew well with good fruit quality, which adapted well to the climate and environment in Binchuan; ＂Little Red Coat＇ showed average performance in growth and major fruit qualities with inferior flavour; ＂Binchuan Red Coat＇ and ＇Binchuan Green Coat＇ also showed average performance in major fruit qualities and and inferior flavour; ＇Acid Pomegranate＇ grew while was inferior in flavour. [Conclusion] This study pro- vides references for presenting the directions for studies of P. granatum L. germplasm resources and determining the genetic relationship among germplasm resources, as well as breeding, introduction and cultivation of P. granatum L., further providing a variety basis for pomegranate production.
