In this paper, we intended to further investigate the effects of small intestine (Ribonucleic acid, RNA), on gene expression of intestines of irradiated mice. This will provide theoretical bases for repair of γ-ray caused damage in small intestine. Twelve BALB/c mice were randomly divided into two groups, one for irradiated con- trol and the other for small intestine RNA therapy (100μg/mL, 0.4mL). Eighteen hours after γ-ray irradiation with a dose rate of 138.82 cGy/min and a total dose of 1150cGy, the mice were killed and their jejunum were taken out. Fluorescent cDNA probes labeled with Cy3 and Cy5 were prepared from RNA extracted from the intestines of the two groups. The probes of the two groups were hybridized against cDNA gene chip, the fluorescent signals were scanned with a scanner and the results were analyzed by a computer codes. Compared with the control group, 47 genes changed in gene expression profile. The expression levels of 13 genes were up-regulated distinctly, while 34 genes were down-regulated significantly. Among the 47 genes, 20 were reported with known or inferred functions, i.e. 8 up-regulated genes (arginase 1, LPS-binding protein, rpS17, IL-18, beta-1-globin,small proline-rich protein 2A, pancreatitis-associated protein,and haptoglobin gene) and 12 down-regulated genes (component 2, integral membrane protein 2, ATP synthase alpha subunit, Man- nose-6-phosphate receptor, IMP dehydrogenase, Gk-rs1, WW-domain binding protein1, ubiquitin fusion degrada- tion1, α1-actin, natural killer cell protein group 2, UDP-glucose dehydrogenase, and EMK2 gene). The other 27 were reported with unknown functions. In conclusions, small intestine RNA could change specific expressions of some genes in mouse small intes- tines and most of these genes were down-regulated ones.
The work is to observe ultrastructure of pituitary gland of rats exposed to electromagnetic pulse (EMP, 200 kV m-1, 200 times)with transmission electron microscope and to explore the effects of EMP on the endocrine system. At 6h and 12h after EMP exposure, the intercellular spaces were dilated, swellen mitochondria with cris- tae loss, dilatation of Golgi complex and diffused lysosomes were found in endocrine cells of pituitary gland, es- pecially in prolactin cells and growth hormone cells. There were macrophages infiltrating in intercellular spaces at 24h after EMP exposure, and more mitochondrial vacuolization in prolactin cells, many myelin figures in growth hormone cells and distinct dilatation of endoplasmic reticulum in gonadotropic cells were found. These changes were gradually aggravated. Numerous secondary lysosomes, the gathering of heterochromatins under the nuclear membranes, and cell degeneration and necrosis could be observed at 48h. These results suggest that EMP may damage endocrine cells in pituitary gland of rats, mitochondria and endoplasmic reticulum are the sensitive organ- elles, and prolactin cells and growth hormone cells are perhaps the sensitive cells to EMP.Effect of electromagnetic pulse (EMP) on ultrastructure of pituitary gland in ratsFANG Henghu1 ZENG Guiying1 REN Dongqing1 JIN Cheng1 GUO Yao1 HUANG Xiaofeng21(Department of Radiation Medicine , Faculty of Preventive Medicine,Fourth Military Medical University, Xi’an 710032) 2(Center of Electron Microscopy, Fourth Military Medical University, Xi’an 710032 ) The work is to observe ultrastructure of pituitary gland of rats exposed to electromagnetic pulse (EMP, 200kV m-1, 200 times)with transmission electron microscope and to explore the effects of EMP on the endocrinesystem. At 6h and 12h after EMP exposure, the intercellular spaces were dilated, swellen mitochondria with cris-tae loss, dilatation of Golgi complex and diffused lysosomes were found in endocrine cells of pituitary gland, es-pecially in prolactin cells and growth ho
