[Objective] This study was to optimize the experimental conditions for large scale propagation of Sagittaria trifolia L via tissue culture.[Method] The dominant S.trifolia cultivar Baoyingziyuan introduced from Jiangsu Province was used as experimental material to study the impacts of various culture conditions on tissue culture of its stem tips and induction of stolons.[Result] Hormone combination 0.10 mg/L 6-BA ＋0.05 mg/L NAA performed best in plantlet regeneration and 2.0 mg/L 6-BA ＋0.5 mg/L NAA best in induction of stolon.Various sucrose concentrations did not show significant difference in the impact on sprouted stolons.[Conclusion] Various culture conditions could to some extent impact plantlet regeneration and stolon induction,and our results reveal the optimal hormone combinations for regeneration and stolon induction of S.trifolia.
[Objective] The aim was to determine the difference in sucrose content of different tartary buckwheat sources,so as to screen low-sucrose buckwheat materials.[Method] The sucrose contents of 35 tartary buckwheat materials from different regions were measured.[Result] The sucrose content of the 35 tartary buckwheat materials varied from 0.007 7% to 0.208 9% with an average of 0.051 8%.There was some difference among the tartary buckwheat materials from different regions.The seeds from Hezhang in Guizhou had higher sucrose content,while the seeds from Nayong in Guizhou had lower sucrose content.[Conclusion] The content of sucrose was significantly low in tartary buckwheat,which could be popularized as a kind of low-sugar crop.
[Objective] This study aimed to finding out the difference in glucose content of tartary buckwheat seeds from different origins.[Method] Taking 35 kinds of tartary buckwheat from different origins as the test materials,the glucose content of seeds had been determined.[Result] The variation range of glucose content in 35 kinds of tartary buckwheat was from 0.055 6% to 0.840 2% with an average of 0.321 7%.There was a difference in glucose content of tartary buckwheat from different origins.The seeds T324 from Weining in Guizhou had the highest glucose content,while the seeds T398 also from Weining in Guizhou had the lowest glucose content.[Conclusion] This study provides a reference for the genetic variation law of glucose content in different tartary buckwheat resources.
