Ecological and cultural factors have strong impacts on the distribution and cultivation of agricultural cultivar., In this paper, the correlation between diversity centers of rice cultivar and ecological, cultural factors was probed, based on datasets of rice cultivar in Yunnan, Southwest China. The results showed that diversity centers of rice cultivar were observed in Southwest Yunnan, South Yunnan and Southeast Yunnan, which may be related to the local culture of rice production and warm, humid climate. For the diversity center in South Yunnan, culture of rice production of Dai and Hani people may play important roles. The diversity center in Southwest Yunnan may relate to the culture of rice production of Dai, Jingpo and Lahu people, and for the diversity center in Southeast Yunnan, Miao, Yao and Zhuang people＇s culture in rice production can not be underestimated. Traditional culture promoted the cultivation of rice cultivars, and high diversity of rice cultivars facilitate the preservation and continuation of the traditional culture as well.
Knowledge of the geographic distribution and frequency of avirulence genes will contribute to the development of strategies to effectively use rice varieties that carry various resistances genes, including combinations of varieties in mixture cropping systems. Here, we analyzed the geographic distribution and frequencies of avirulence genes in rice blast fungus using samples collected from 11 prefectures across Yunnan province, China. A total of 467 single spore isolates were assayed for pathotypes based on their reaction to 20 rice blast resistance monogenic lines. The results revealed that frequencies of avirulence genes among 10 prefectures showed insignificant difference, but frequencies of avirulenee genes in Xishuangbanna showed significant differences compared to the remaining 10 prefectures. The avirulence genes Avr-Pi9, Avr-Piz and Avr-Pizt were observed at the highest frequency in blast isolates from the 11 prefectures; their average frequency was greater than 80%. Our results imply that the composition and distribution of rice genetic diversity are more important than climate and other environment conditions for formation and maintenance of rice blast fungus genetic diversity. Using average frequencies, the avirulence genes can be categorized into 4 groups. There were significant differences of frequencies of avirulence genes among different groups, while insignificant differences observed within any group. These results will provide useful information for evaluation of resistance genes and effective management of rice blast disease.
