To investigate the effects and to elucidate the mechanism of Chinese herbs Recipe(consisting of Rhizoma Atractylodis Macrocephalae, Radix Salviae Simplicifoliae, Fructus Aurantii, et al) on antagonizing alcoholic liver injury from the pathological relationship between alcoholic liver injury, gut permeability and endotoxin leakage. The SD rats were fed the Lieber- Decarli liquid alcohol die(t35% of calories from alcohol)to deveclp the alcoholic liver injury model. 42 male SD rats were divided into 4 groups:normal group(n=6),no- alcohol liquid diet group in which alcohol was replaced with carbohydrates(Control group, n=12), alcohol liquid diet group(alcohol group, n=12) and alcohol liquid eiet with Chinese herbs group(herbs group, n=12). Starting from the third week, the rats were fed distilled water, no- alcohol liquid diet, alcohol liquid diet or alcoholic liquid diet with Chinese herbs by intragastric infusion for 6 weeks. Then the rats were fast for 5 h. After that, each group received 10 mg/kg of endotoxin(lipidopolysacchride, LPS)by intragastric infusion. 3.5 hours later, the blood from portal vein and vena cava was harvested and liver biopsy was performed to analyze:1. The histopathological change in small intestine (HE staining and electron microscopic observation); 2. The histopathological change in liver(HE staining, oil red O staining); 3. The TG and r- GT activities in liver and the ALT, AST activities in serum; 4. The plasma endotoxin level in portal vein blood; 5. The protein expressions(on Wetern blot) of P- I k B and TNF- a in liver.Results: that It is 1.Fatty degeneration was seen in over 1/3 of the heptocytes in the alcohol group. The liver TG level in this group was about 7.3 times higher than that in the normal group and 4.9 times higher than that in the control group. In addition, the serum ALT, AST activities as well as liver r- GT activity in the alcohol group were significantly elevated than those in the normal and control group. Observation under electron microscopy revaled swelling, degene
Objective： To study the intervention effects of Jianpi Liqi Huoxue Decoction （健脾理气活血汤, JLHD） on lipid peroxidative liver injury induced by alcohol. Methods： The rat alcoholic model of liver disease （ALD） induced by Lieber-DeCarli liquid diet was established. Thirty-two male SD rats were randomly divided into 4 groups ： the normal group （ n = 5）, the control group （ n = 9）, the model group （ n = 9） and the JLHD group （ n =9）. From the 4th week after modeling, the rats were given JLHD or distilled water by gastrogavage respectively, and the samples of blood and liver tissues were taken out from the rats for determination by the end of the 8th week. The hepatic pathological changes were observed with HE staining; the liver injury related indices, including activity of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in serum, γ-glutamyl transpeptidase （γ-GT） activity and triglyceride （TG） content in liver tissues, as well as the lipid peroxidation related indices, including malonaldehyde （MDA） content and nitric oxide synthase （NOS） activity in liver tissue, serum Fe^2＋ level, and the anti-peroxidation capacity related indices, including superoxide dismutase （SOD） activity, glutathion （GSH） content and reactive oxygen species （anti- ROS） activity in liver tissues were determined. Results： （ 1 ） There were obvious figures of fatty degeneration and inflammatory infiltration in liver tissues of the model group. As compared with the control group, in the model group, the activity of ALT and AST, and Fe^2＋ content in serum, γ-GT and NOS activity, TG and MDA content in liver tissues were significantly higher （ P〈0. 01 ）, while the activity of SOD, GSH and anti-ROS in liver tissues were significantly lower （P〈0.01）. （2） The fatty degeneration and inflammatory infiltration of liver tissues in the JLHD group were significantly lessen as compared with those in the model group; and the abnormalities o
Objective:To evaluate the effect of Jianpi Huoxue decoction(健脾活血方,JHD)-containing serum on tumor necrosis factor-α(TNF-α) secretion and endotoxin receptor gene expression in RAW264.7 cells induced by lipopolysaccharide(LPS).Methods:The cytotoxicity of blank-control serum and JHD-containing serum at different concentrations were evaluated through the lactate dehydrogenase(LDH) assay in RAW264.7 cells.RAW264.7 cells were divided into six groups:5%blank-control serum group(C1,n=3),5%blank-control serum plus LP...
