In plant cells, the cytoskeletons play important roles in the responses to biotic and abiotic stresses. Howeve...
Hai- Yong Yuan, Lin-Lin Yao, Zhi-Qi Jia and Ying-Zhang Li (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094)
Characterization of defense genes should permit greater understanding of defense response mechanisms at the mo...
Yan-Yan Hou Ying-Zhang Li (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094
Cotton Verticillium wilt caused by Verticillium dahliae is one of the major diseases, which seriously affect t...
Zhi-Qi Jia, Hai-Yong Yuan, Yan-Yan Hou, Fu-Mei Shi and Ying-Zhang Li (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China)
The complexes excreted by VerticiUium dahliae are phytotoxins, which are responsible for most of the symptoms associated with Verticillium wilt disease. Verticillium dahliae toxins (VD-toxins) can be purified by different methods. In the present study, we reported a simpler, more effective method to purify VD-toxins. The supematant of V. dahliae culture was frozen, lyophilized and dialyzed by 1 kDa Dialysis Membranes (MWCO). We also partially identified the characteristics of the purified VD-toxins. The results showed that the components of VD-toxins include glycoprotein within 35.8-83.2 kDa. The phytotoxic activity of VD-toxins was remained after VD-toxins were pretreated by high temperature, Concanavalin-A, and proteinase E, respectively. These data suggest that VD-toxins are heat-stable, and the protein fraction and glycosyl are both important contributors to the phytotoxic activity. VD-toxins purified effectively from the culture filtrates of V. dahliae may help in further understanding the mechanisms of interactions between V. dahliae and plants.
JIA Zhi-qi SHI Fu-mei YUAN Hai-yong HOU Yan-yan LI Ying-zhang