The porcine microsatellite SW943 was regionally localized on 12p11-(2/3p13) by the two methods: the Primed in situ (PRINS) labelling on the pachytene bivalents of pigs using the Dig-11-dUTP as the report molecule and pig X rodent Somatic Cell Hybrid Panel (SCHP) which contains 27 cell lines through PCR amplification. Advantages and disadvantages of the two methods for physical mapping of microsatellites were also discussed.
LIU Bang, WANG Yong-qiang, ZHANG Qing-de, YU Mei, ZHAO Shu-hong, XIONG Tong-an and LI Kui(Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070 ,P. R . China)
