This review summarized the recent research achievements on the process of tissue culture of the rare and endangered plant Elaeagnus mollis,including sterilizing protocols of different explants derived from adult plants and seeds for tube germination,two types of plants regeneration(organ type and organ genesis type),methods of rooting and transplanting,factors affecting culture,as well as browning and vitrification phenomena and avoiding measures.And the further biotechnology research fields of E.mollis were prospected.