[Objective ] The study aimed to construct the AFLP molecular marking system in Mangifera indica. [ Method ] Four varieties of Mangifera indica were used to explore new ways for high-quality DNA, and AFLP analysis of 31 varieties of Mangifera indica was carried out to detect the varietal genetic diversity. [ Result] 14 pairs of primers with stronger polymorphism, better banding patterns and higher resolution were screened out from 64 pairs of selective amplification primers. Then they were used to analyse the fingerprint of 31 varieties of Mangifera indica, the results showed that the ratio of polymorphic bands amplificated by the 14 pairs of primers reached 97% in 31 varieties of Mangifera.[ Conclusion] It was suggested that AFLP was suitable for detecting the polymorphism of Mangifera indica resources.
Ostryopsis davidiana is a special species in China, and is also advantagous shrub of Daqing Mountains weak ecological system; Cenococcum geophilum (Fr.) spread widly in the world and have strong adaptability. C.geophilum (Fr.) applied to experiments can be isolated from O.davidianas thin roots. It can be proved by seedling back-inoculation to O.davidiana that inoculated mycorrhizae infectious rate reaches to 67.2, and that formed mycorrhiza is the same as wild mycorrhiza. It was found that the typical sclerotium organs in seedling back-inoculation substrate. The experiments prove that C.geophilum(Fr.) has no obvious affect on O.davidianas overground parts, however, it has apparent promotion to growth of ground iameter and roots. Inoculation disposal root/shoot ratio is 2.18 times more than comparative disposal one. This may be the reason that O.davidiana can grow normally while other plants on Daqing Mountain become degraded and extinct.
