Clonorchiasis,caused by Clonorchis sinensis(C.sinensis),is an important food-borne parasitic disease and one of the most common zoonoses.Currently,it is estimated that more than 200 million people are at risk of C.sinensis infection,and over 15 million are infected worldwide.C.sinensis infection is closely related to cholangiocarcinoma(CCA),fibrosis and other human hepatobiliary diseases;thus,clonorchiasis is a serious public health problem in endemic areas.This article reviews the current knowledge regarding the epidemiology,disease burden and treatment of clonorchiasis as well as summarizes the techniques for detecting C.sinensis infection in humans and intermediate hosts and vaccine development against clonorchiasis.Newer data regarding the pathogenesis of clonorchiasis and the genome,transcriptome and secretome of C.sinensis are collected,thus providing perspectives for future studies.These advances in research will aid the development of innovative strategies for the prevention and control of clonorchiasis.
目的探讨华支睾吸虫重组14-3-3ε蛋白(r Cs14-3-3ε)对肝内胆管癌细胞株CCLP-1细胞波形蛋白(vimentin)表达的影响,观察该过程中CCLP-1细胞侵袭和迁移能力的变化。方法用不同浓度(1、5、10μg/ml)r Cs14-3-3ε与CCLP-1细胞共孵育24、48、72 h后,通过Q-PCR、Western blot等实验方法检测vimentin的表达水平。用划痕愈合实验和Transwell小室侵袭实验检测r Cs14-3-3ε对CCLP-1细胞迁移和侵袭能力的影响。结果 Q-PCR实验表明5μg/ml r Cs14-3-3ε刺激CCLP-1细胞72 h后及10μg/ml r Cs14-3-3ε刺激CCLP-1细胞24、48、72 h后,vimentin m RNA水平显著上调;Western blot实验显示,10μg/ml r Cs14-3-3ε刺激CCLP1细胞48、72 h后,vimentin蛋白水平显著上调。划痕实验表明,r Cs14-3-3ε(10μg/ml)能够促进细胞的迁移能力,处理后的细胞迁移率增大(P<0.01);Transwell小室检测显示,r Cs14-3-3ε(10μg/ml)处理组中穿膜细胞数为对照组的1.78倍,显著增加(P<0.05)。结论 r Cs14-3-3ε可以通过上调vimentin蛋白的表达,从而促进肝内胆管癌细胞株CCLP-1的侵袭和迁移能力。
