Objective： To determine whether the nodule and eminence on the frenulum labii superioris, location of Yinjiao （DU28） according to the meridian theory, could be used to prognosticate the recurrence of patients with colorectal cancer. Methods： The data of 101 patients with colorectal cancer in Tianjin Medical University Cancer Institute and Hospital from May 2011 to November 2011 was analyzed. The photos of all patients＇ frenulum labii superioris were taken. Nodule and eminence on frenulum labii superioris were the positive standard. Biopsy and pathological testing for the nodule were carried out on one patient with large nodule on frenulum labii superioris. Results： Patients with positive frenulum labii superioris had a higher risk of recurrence and/or metastasis than patients with negative frenulum labii superioris. There were no significant differences in diagnosis of recurrence and/or metastasis between the status of frenulum labii superioris and the traditional diagnostic criteria （P=0.238）. The Kappa was 0.606 （P〈0.001）. The sensitivity was 76.0% and the specificity was 85.4%. The pathological report demonstrated that the nodule on frenulum labii superioris was mucosal excrescence with normal structure. Conclusion： Nodule and eminence on frenulum labii superioris are potential diagnostic markers for metastatic colorectal cancer.
目的观察褐藻多糖对人乳腺癌细胞株MCF-7、MDA-MB-231细胞活性的影响,并探讨其作用机制。方法将对数生长期的MCF-7、MDA-MB-231细胞(观察A组、B组)分别加入100、200μg/m L褐藻多糖2 m L,对照A组、B组均加入等体积的DMEM培养基2 m L。用台盼蓝染色实验测算细胞增殖抑制率,用流式细胞仪检测细胞周期和凋亡细胞,用Western blot法检测细胞Caspase-8及磷酸化细胞外调节蛋白激酶(p-Erk)、Erk表达。结果随着褐藻多糖浓度增加、作用时间延长,观察A组、B组细胞增殖抑制率逐渐升高(P均<0.05);观察A组、B组G0/G1期细胞比例升高、S期及G2期细胞比例降低,细胞凋亡率增高,褐藻多糖浓度高者变化更明显,P均<0.05;观察A组、B组细胞Caspase-8表达升高、p-Erk表达下降,褐藻多糖浓度高者变化更明显,P均<0.05。结论褐藻多糖可抑制MCF-7、MDA-MB-231细胞增殖,阻滞细胞周期于G0/G1期,促进细胞凋亡;该作用可能是通过上调细胞Caspase-8表达、下调p-Erk表达水平实现。
Objective： To evaluate the diagnostic significance of sublingual nodules for metastasis of patients with breast cancer and further to explore the mechanisms of sublingual nodules. Methods： The image data of 117 in-patients with breast cancer in stage I -IV in Tianjin Medical University Cancer Institute and Hospital from December 2009 to September 2011 were assessed retrospectively. All photos of patients＇ tongue were recorded by the digital camera of uniform type within 1 month after serological examination and regular re-examined by computed tomography （CT）, magnetic resonance imaging and positron emission tomography CT. The presence of sublingual nodules was the positive standard. Chi square test and two-independent-sample test were used to determine the diagnostic value between the status of sublingual nodules and Clinico-pathological characteristics. The optimal cut-off of uric acid （UA） level to diagnose sublingual nodules was determined by receiver operating curve （ROC） analysis. Results： Breast cancer patients with sublingual nodules had a higher risk of recurrence and/or metastasis than patients without it （P〈0.001）. Sublingual nodules was significantly correlated with increased serum UA level （P=0.001）. The optimal cut-off value of UA level to diagnose sublingual nodules was 290 μ mol/L. Furthermore, the elevated serum UA level （ ≥290 μmol/L） was significantly related to breast cancer recurrence and/or metastasis （P〈0.001）. Couclusions： Sublingual nodules were potential diagnostic markers for metastatic breast cancer. The formation of sublingual nodules was associated with elevated level of serum UA.
