The techniques used in the field of drug discovery have been quickly developed during the past decades High throughput screening (HTS) is a new technique in drug discovery with the characteristics of efficiency, huge scale and automatically operation The assay methods used in HTS are based on the molecular targets and cells Screening results obtained from these methods indicate not only the bioactivities of compounds (samples) but also the mechanisms of actions of the compounds (samples) HTS has altered the strategy of drug
cAMP反应元件结合蛋白(cAMP response elem ent b ind-ing prote in,CREB)是位于细胞核内的转录因子。CREB的活性受到信号通路中许多因子的调控。CREB活化后与真核生物靶基因CRE序列结合并调节其转录,发挥多种生物学效应。在中枢神经系统,CREB调节着神经细胞生长发育,参与神经细胞突触可塑性、长时程记忆的形成过程。CREB参与阿尔采末病、血管性痴呆、亨廷顿舞蹈病及H IV-相关痴呆等神经退行性疾病的病理生理机制研究也取得了进展,成为以CREB作为靶点控制神经退行性疾病病程的理论基础。
The experimental models for drug screening are very important points in drug discovery Although the drug screening techniques have been developed, such as high throughput screening (HTS), the screening assay methods (models) still limited drug discovery In present paper, advanced animal models, cell assays and molecular methodology used for drug discovery were reviewed The characteristics and requires of the assay methods used for drug discovery in HTS were also
A variety of technologies continue to develop for high throughput screening This review summarized recent advances of bioassay techniques used in high throughput screening The principles, applications, characteristics and other parameters of these methods were described such as homogeneous time resolved fluorescence, fluorescence polarization, fluorescence resonance energy transfer, fluorescence correlation spectroscopy and scintillation proximity
