We collected fish abundance data in the Changjiang(Yangtze River) estuary and adjacent waters in November 1998,May 1999,November 2000,and May 2001.Using the data,we evaluated the characteristics of the fish assemblages at each site and investigated the effect of several environmental factors.We used a multivariate analysis,including community ordination methods such as detrended correspondence analysis(DCA) and canonical correspondence analysis(CCA),and two-way indicator species analysis(TWINSPAN).We analyzed the biological community structure and environmental factors to determine their spatial distributions,temporal dynamics,and seasonal variations.Among the fish species,five exceeded 5% of the total abundance:Harpodon nehereus(42.82%),Benthosema pterotum(13.85%),Setipinna taty(11.64%),Thryssa kammalensis(9.17%) and Apogonichthys lineatus(6.49%).These were separated into four ecological assemblages:hypsithermal-saline,hypsithermal-brackish,hypothermal-brackish,and hypothermal-saline.We evaluated the degree of influence of environmental factors on the fish community.Our analyses suggested that environmental factors including water depth,salinity,turbidity,transparency,nutrient,and suspended matter formed a synthetic spatial gradient between the coastal and pelagic areas.Ecological and environmental factors changed temporally from 1998 to 2001,and drove the fish community succession.The environmental factors driving the fish community structure included bottom temperature,water depth,bottom and surface pH,surface total phosphorous,and bottom dissolved oxygen.This investigation was completed before completion of the Three Gorges Dam;therefore the results of this study provide an important foundation for evaluating the influence of the human activities.
Filtration and cross-flow ultrafiltration techniques were used to separate culture media of Prorocentrurn donghaiense at the exponential growth, stationary and decline stages into 〈0.45 μm filtrate, 100 kDa-0.45 μm, 1%100 kDa and 1-10 kDa retentate and 〈1 kDa ultrafiltrate fractions. The fluorescence properties of different molecular weights of dissolved organic matter （DOM） were measured by excitation-emission matrix spectra. Protein-like and humic-like fluorophores were observed in the DOM produced by P. donghaiense. The central positions of protein-like fluorophores showed a red shift with prolonged growth duration, shifting from tyrosine-like properties at the exponential growth stage to tryptophan-like properties at the stationary and decline stages. The excitation wavelengths of protein-like fluorophores exhibited some change in the exponential growth and stationary stages with increased molecular size, but showed little change in the decline stage. However, the emission wavelengths in the decline stage exhibited a blue shift. Very distinct C type and A type peaks in humic-like fluorophores were observed. With a prolonged culture time, the intensities of both of the peaks became strong and the excitation wavelengths of peak A showed a red shift, while the A：C ratios fell. More than 94% of fluorescent DOM was in the lower than 1 kDa molecular weight fraction.
