目的通过microRNA芯片筛选不同基底刚度影响宫颈癌细胞迁移过程中的关键microRNA的候选者。方法用丙烯酰胺及双丙烯酰胺的不同比例配置基底刚度为1、20 k Pa的人工基底膜,在其上种植宫颈癌细胞系Siha,36 h后收取细胞提取总RNA,用microRNA芯片及qRT-PCR方法筛选2种基底刚度条件下的差异表达microRNA。结果在2种不同刚度条件下生长的宫颈癌细胞系Siha共有173个表达差异的microRNA,其中包括70个表达上调的microRNA和103个表达下调的microRNA。在宫颈癌细胞系Siha、Hela、Caski中,检测与宫颈癌细胞迁移相关的miR-21、miR-125a、miR-75b、miR-150、miR-595、miR-218、miR-200b、miR-107、miR-183等表达与microRNA芯片结果一致。结论不同基底刚度可引起宫颈癌细胞系Siha的microRNA表达谱不同,microRNA在基底刚度调控宫颈癌细胞迁移的过程中可能具有重要作用。
Objective： The high expression of cell division cycle 42 protein （CDC42） may be involved in the occurrence and progression of several tumors. However, the expression and function of CDC42 in cervical squamous cell carcinoma remains unclear. This study aimed to investigate the expression of CDC42 in cervical squamous cell carcinoma and its correlation with clinicopathologic characteristics. Methods： The expression of CDC42 in 162 cervical squamous cell carcinoma tissue samples and 33 normal cervical tissue samples was investigated by immunohistochemistry. The CDC42 mRNA expression was detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results： The cervical squamous cell carcinoma group showed a significantly higher CDC42 positive rate, compared to the normal cervical tissues （P〈0.05）. Fttrthermore, the tissues of stage Ⅱ-Ⅳ carcinoma patients showed higher CDC42 expression levels compared to stage I patients （P=0.05）. In addition, the expression of CDC42 was not correlated to age of patients, differentiation degree of cancer cells, or lymph node metastasis （P〉0.05）. Furthermore, compare with normal cervical tissues, the CDC42 mRNA expression in cervical cancer had no significant difference. Conclusions： CDC42 was up-regulated at protein level, but not mRNA level, in cervical squamous cell carcinoma. The high expression of CDC42 was correlated to the clinical stage of the patients, indicating that CDC42 might contribute to the progression of cervical squamous cell carcinoma.
Ding MaYuan ChengYouyi ZhangYanli GuoZijian LiGeng Li
