您的位置: 专家智库 > >

国家自然科学基金(20971034)

作品数:12 被引量:17H指数:2
相关作者:张金超李亚平郝晓红孙静张群更多>>
相关机构:河北大学更多>>
发文基金:国家自然科学基金河北省自然科学基金留学人员科技活动项目择优资助经费更多>>
相关领域:医药卫生理学生物学化学工程更多>>

文献类型

  • 12篇中文期刊文章

领域

  • 4篇医药卫生
  • 4篇理学
  • 3篇生物学
  • 1篇天文地球
  • 1篇化学工程

主题

  • 6篇分化
  • 6篇MINERA...
  • 5篇原代培养
  • 5篇细胞
  • 5篇OSTEOB...
  • 5篇DIFFER...
  • 5篇成骨
  • 5篇PROLIF...
  • 4篇增殖
  • 4篇矿化
  • 4篇成骨细胞
  • 3篇稀土
  • 3篇骨细胞
  • 3篇MOUSE
  • 2篇稀土离子
  • 2篇离子
  • 2篇RARE_E...
  • 2篇成骨分化
  • 1篇英文
  • 1篇稀土化合物

机构

  • 6篇河北大学

作者

  • 6篇张金超
  • 4篇李亚平
  • 3篇郝晓红
  • 3篇孙静
  • 3篇王书香
  • 3篇张群
  • 2篇杨康宁
  • 1篇谷广其
  • 1篇葛昆
  • 1篇刘丹丹

传媒

  • 6篇无机化学学报
  • 5篇Journa...
  • 1篇Scienc...

年份

  • 1篇2016
  • 4篇2012
  • 2篇2011
  • 5篇2010
12 条 记 录,以下是 1-10
排序方式:
Effect of yttrium ion on the proliferation,differentiation and mineralization function of primary mouse osteoblasts in vitro被引量:2
2010年
A series of experimental methods including MTT test,alkaline phosphatase(ALP) activity measurement,oil red O stain and measurement and mineralized function were employed to assess the effects of Y3+ on the proliferation,differentiation,adipogenic transdifferentiation and mineralization function of primary mouse osteoblasts(OBs) in vitro.The results indicated that Y3+(1×10-9,1×10-8,1×10-7,1×10-6,1×10-5,and 1×10-4 mol/L) promoted the proliferation of OBs on day 1,2 and 3.Y3+ had no effect on the differentiati...
张金超刘翠莲李亚平孙静王鹏邸科前陈航赵燕燕
关键词:OSTEOBLASTSPROLIFERATIONDIFFERENTIATIONMINERALIZATION
Effects of Nd^(3+) and Sm^(3+) on the proliferation,differentiation and mineralization function of primary osteoblasts in vitro被引量:1
2010年
A series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) test,alkaline phosphatase (ALP) activity measurement,Oil Red O stain and measurement,mineralized function expression and quantitive real time RT-PCR (qRT-PCR) were employed to assess the effect of Nd3+ and Sm3+ on the proliferation,differentiation and mineralization function of primary osteoblasts (OBs) in vitro at cell and molecular levels.The experimental results suggest that concentration,culture time and ion species are pivotal factors for switching the biological effects of rare earth ions from toxicity to activity,from damage to protection,or from down-regulation to up-regulation.
ZHANG JinChaoSHANG MiQinZHANG DaWeiLI YaPingSUN JingCHEN Hang
关键词:PCR检测
Gd^(3+)对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响(英文)被引量:1
2011年
本文采用MTT法、碱性磷酸酶活性测定、矿化功能的测定以及油红O的染色和定量测定等手段研究了Gd3+对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响。研究结果表明,浓度为1×10-10和1×10-8 mol.L-1的Gd3+对小鼠骨髓基质细胞的增殖没有影响,其他测试浓度下的Gd3+则抑制小鼠骨髓基质细胞的增殖。当Gd3+与小鼠骨髓基质细胞作用7 d时,其对小鼠骨髓基质细胞成骨分化的影响与作用浓度有关,当Gd3+与小鼠骨髓基质细胞作用14 d时,在全部测试浓度范围内,抑制小鼠骨髓基质细胞成骨分化。除1×10-8和1×10-5 mol.L-1外,其他测试浓度下的Gd3+促进小鼠骨髓基质细胞的矿化功能。当Gd3+与小鼠骨髓基质细胞作用10 d时,其抑制小鼠骨髓基质细胞的成脂分化,当Gd3+与小鼠骨髓基质细胞作用16 d时,除1×10-9mol.L-1外,其他浓度的Gd3+也抑制小鼠骨髓基质细胞的成脂分化。实验结果提示,Gd3+可能通过促进骨髓基质细胞的成骨分化、抑制其成脂分化途径起到对骨的保护作用。Gd3+对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响与作用浓度和时间有关,而且,它们是影响Gd3+对骨是损伤还是保护作用转变的关键因素。
张金超谷广其孙静张群郝晓红王书香
关键词:稀土离子骨髓基质细胞成骨分化成脂分化
稀土化合物TbCl3对成骨细胞系MC3T3-E1增殖、分化和矿化功能的影响被引量:2
2016年
在细胞和分子水平上,研究了稀土化合物氯化铽(TbCl_3)对成骨细胞MC3T3-E1增殖、分化及矿化功能的影响。结果表明,细胞水平上,浓度为0.000 1、0.001、0.01、0.1、1和10μmol·L-1的TbCl_3均促进MC3T3-E1细胞的增殖、分化及其矿化功能,然而,当浓度升至为100和1 000μmol·L-1时,TbCl_3表现出抑制作用。分子水平上,浓度为0.000 1和0.1μmol·L-1的TbCl_3明显上调成骨分化相关基因骨形成蛋白2(BMP-2),碱性磷酸酶(ALP),骨涎蛋白(BSP),Ⅰ型胶原蛋白(ColⅠ),骨钙素(OCN)和runt相关转录因子2(Runx2)的表达。浓度为1 000μmol·L-1的TbCl_3则抑制上述成骨分化相关基因的表达。浓度为0.000 1、0.1和1μmol·L-1的TbCl_3促进成骨分化相关蛋白Runx2,BMP-2和OCN的表达;结果显示,低浓度的TbCl_3促进MC3T3-E1细胞的成骨分化及矿化功能,而高浓度TbCl_3则呈现出抑制作用。TbCl_3通过调控Runx2的表达刺激早期成骨分化相关基因BMP-2、ColⅠ和晚期成骨分化相关基因ALP、OCN的表达,从而诱导MC3T3-E1成骨分化。
刘丹丹葛昆孙静张少瀚张金超
关键词:增殖成骨分化矿化
Effect of cerium ion on the proliferation,differentiation and mineralization function of primary mouse osteoblasts in vitro被引量:1
2010年
The effects of cerium ion(Ce3+) on the proliferation,differentiation,adipocytic transdifferentiation and mineralization function of primary mouse osteoblasts(OBs) were investigated.The results indicated that Ce3+ at all concentrations(1×10-9,1×10-8,1×10-7,1×10-6,1×10-5,and 1×10-4 mol/L) promoted the proliferation of osteoblasts(OBs).On day 1 and 3,Ce3+ promoted the differentiation of OBs at concentrations of 1×10-9,1×10-7,and 1×10-6 mol/L,but inhibited the differentiation of OBs at higher concentrations.On ...
张金超刘翠莲李亚平孙静王鹏邸科前赵燕燕
关键词:OSTEOBLASTSPROLIFERATIONDIFFERENTIATIONMINERALIZATION
硝酸锶对原代培养的小鼠成骨细胞增殖、分化和矿化功能的影响(英文)
2012年
采用噻唑蓝(MTT)法、碱性磷酸酶(ALP)比活性测定、油红O染色、Ⅰ型胶原测定以及矿化结节染色及定量分析等方法,研究了不同浓度的硝酸锶对原代培养的成骨细胞增殖、分化、矿化功能以及横向分化为脂肪细胞的影响。结果表明:硝酸锶对成骨细胞增殖、分化、矿化功能以及横向分化为脂肪细胞的影响与作用浓度和时间密切相关,但没有呈现出剂量依赖性。结果提示,硝酸锶对骨代谢的影响是复杂的,其具有保护还是损害作用取决于作用浓度和时间,而且它们是影响硝酸锶生物效应(从损伤到保护)转变的关键因素。
张金超郝晓红张群李亚平王书香
关键词:硝酸锶成骨细胞增殖分化矿化
Effects of Er^(3+) on the proliferation,differentiation and mineralization function of primary mouse osteoblasts in vitro被引量:2
2011年
A series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) test,alkaline phosphatase (ALP) activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR (qRT-PCR) were employed to assess the effects of Er3+ on the proliferation,differentiation and mineralization function of primary osteoblasts (OBs) in vitro at cell and molecular levels. The results indicated that Er3+ inhibited the proliferation of OBs at a concentration of 1×10–7 mol/L,but had no effect at other concentrations. Er3+ inhibited the differentiation of OBs at concentrations of 1×10–8,1×10–7,and 1×10–6 mol/L,but had no effect at a higher concentration of 1×10–5 mol/L. Er3+ had no effect on the transdifferentiation of OBs at tested concentrations. Er3+ inhibited the mineralization function of OBs at concentrations of 1×10–7,1×10–6,and 1×10–5 mol/L,but had no effect at a lower concentration of 1×10–8 mol/L. The expression of the mRNA for runt-related transcription factor 2 (RUNX-2) and peroxisome proliferators activated receptor γ (PPAR-γ) was down-regulated in the presence of 1×10–6 mol/L Er3+. These findings suggested that Er3+ might have negative effect on bone metabolism.
张金超孙静张大威李亚平郝晓红秦新英
关键词:OSTEOBLASTSPROLIFERATIONDIFFERENTIATIONMINERALIZATION
DyCl_3对原代培养的成骨细胞增殖、分化和矿化功能表达的影响(英文)
2010年
采用MTT法、碱性磷酸酶活性测定、油红O的染色和定量测定、矿化功能的测定以及qRT-PCR等手段在细胞和分子水平上研究了DyCl3对原代培养的成骨细胞增殖、分化和矿化功能的影响。研究结果表明,在测试浓度范围内,DyCl3均抑制成骨细胞增殖。浓度为1×10-8,1×10-6和1×10-5mol·L-1的DyCl3促进成骨细胞分化。在测试浓度范围内,DyCl3均抑制成骨细胞横向分化为脂肪细胞。浓度为1×10-5mol·L-1的DyCl3促进成骨细胞矿化结节的形成,而浓度为1×10-7mol·L-1和1×10-6mol·L-1的DyCl3抑制成骨细胞矿化结节的形成,进一步降低浓度为1×10-8mol·L-1,它则对成骨细胞矿化功能没有影响。浓度1×10-6mol·L-1的DyCl3显著降低PPAR-γmRNA表达水平,但相同浓度DyCl3则显著上调RUNX-2mRNA表达水平。实验结果提示,DyCl3对体外培养的成骨细胞增殖、分化及矿化功能的影响与浓度和作用时间有关,而且,它们是影响其生物效应从毒性到活性,从损伤到保护,从上调到下调转变的关键因素。这些结果对深入理解稀土离子对骨代谢的影响具有重要的价值。
张金超杨康宁孙静李亚平
关键词:稀土离子增殖分化矿化
Effects of La^(3+) on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells被引量:2
2012年
In order to elucidate the action of La3+ on bone metabolism,effects of La3+ on the osteogenic and adipogenic differentiation of pri-mary mouse bone marrow stromal cells(BMSCs) were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) test,alkaline phosphatase(ALP) activity measurement,mineralized function,oil red O stain and measurement.The results showed that La3+ pro-moted the proliferation of BMSCs except at 1×10-10 and 1×10-6 mol/L.The effect of La3+ on the osteogenic differentiation depended on con-centrations at the 7th day,but the osteogenic differentiation was inhibited at any concentration at the 14th day.La3+ promoted the formation of mineralized matrix nodules except at 1×10-8 and 1×10-5 mol/L.La3+ inhibited adipogenic differentiation except at 1×10-10 and 1×10-7 mol/L at the 10th day,and inhibited adipogenic differentiation except at 1×10-9 mol/L at the 16th day.These findings suggested that La3+ might have protective effect on bone at appropriate dose and time.This would be valuable for better understanding the mechanism of the effect of La3+ on bone metabolism.
ZHANG JinchaoSUN JingGU GuangqiHAO XiaohongLIU DandanLI YapingQIN XinyingWANG Shuxiang
关键词:MINERALIZATION
Cu^(2+)和Cu^+对原代培养的小鼠成骨细胞增殖、分化和钙化的影响(英文)被引量:6
2010年
利用噻唑蓝(MTT)法、碱性磷酸酶(ALP)比活性测定、油红O染色和矿化结节染色及定量分析,研究了Cu2+和Cu+对原代培养的成骨细胞增殖、分化及钙化的影响。结果显示:Cu2+(1×10-9~1×10-6 mol.L-1)促进成骨细胞增殖,随时间延长,促进作用变弱。Cu+(1×10-7~1×10-5 mol.L-1)抑制成骨细胞增殖,随时间延长,浓度为1×10-6 mol.L-1的Cu+为促进作用,其余浓度则没有影响。对于成骨细胞分化,Cu2+和Cu+表现出相似的影响,浓度为1×10-9和1×10-6 mol.L-1时均促进成骨细胞分化,而当浓度为1×10-7和1×10-5mol.L-1时,则抑制成骨细胞分化,随作用时间延长,大多数浓度均表现为促进作用。测试浓度下的Cu2+和Cu+均对成骨细胞向脂肪细胞的横向分化表现为促进效应。对矿化功能的影响,1×10-5mol.L-1的Cu2+和Cu+表现出显著的抑制效应,但随浓度降低,抑制效应变弱。1×10-7 mol.L-1的Cu2+促进成骨细胞矿化结节的形成。结果提示:作用浓度、作用时间及铜离子的价态都是影响Cu2+和Cu+生物效应转变(从毒性到活性,从损伤到保护,从下调到上调)的关键因素。
张金超李亚平杨康宁郝晓红
关键词:成骨细胞分化钙化
共2页<12>
聚类工具0