[Objective] The research aimed at investigating the taxonomic position and phylogenetic relationship of bisexual brine shrimps.[Method] 16S rDNA of three species of bisexual Artemia from China was determined;the homologous sequences between them and 11 relative species of Artemia from GenBank were compared;the molecular phylogenetic tree was constructed by Mega Microsoft using Artemiopsis stefanssoni as outgroup.[Result] Artemia persimilis was the primal group in genus Artemia;Artemia franciscana and Artemia monica were the evolved groups;Artemia urmiana,Artemia sinica and other Artemia species from China shared a close genetic relationship.[Conclusion] Based on the 16S rDNA sequence of them,the phylogenetic relationships of these bisexual Artemia species were A.persimilis→A.urmiana,A.sinica and A.tibetiana→A.tunisiana→A.monica→A.Franciscan.
本文对土尔蝗属Tuarega Uvarov,1943进行了分类研究,记述了撒哈拉地区2新种——撒哈拉土尔蝗Tuaregasahara sp.nov.和巴氏土尔蝗Tuarega parisi sp.nov.,并编制了该属3个已知种的检索表。新种模式标本保存于西班牙国立自然博物馆(Museo Nacional de Ciencias Naturales)。
[Objective] The aim was to explore the phylogenetic relationships among four subspecies of the genus Locusta.[Method] The sequences of three subunits of cytochrome oxidase of Locusta migratoria tibetensis and Locusta migratoria manilensis were amplified and sequenced（COⅠ 1 539 bp,COⅡ 684 bp,CO Ⅲ 792 bp,with the total of 3 015 bp）.The corresponding sequenses of Locusta migratoria migratoria and Locusta migratoria migratorioides were obtained from GenBank and constructed a multiple alignment.Phylogenic trees of four subspecies of L.migratoria were constructed by Neighbor-Joining,Maximum-parsimony and Bayesian,respectively.[Result] The average content of A ＋ T in three subunits of four subspecies was 69.57%;the third site of codon showed the highest A ＋ T content,and the COⅠ had the highest A ＋ T content（87.6%）;The nucleotide substitution mainly occurred at the third site of codon,and the nucleotide replacement rate of CO Ⅱ was the highest.The second site of codon was conservative,so the replacement rate was in the range of 5.9%-15%.The start codon of COⅠ was CCG or ACG.Genetic distances among four subspecies were ranged from 0.001 to 0.076.The relationship between L.m.tibetensis and Locusta migratoria manilensis was the closest,followed by L.m.migratorioides and L.m.migratorioides,while the genetic distance between L.m.tibetensis and L.m.migratorioides was the largest.[Conclusion] The phylogenetic relationships among four subspecies of Locusta migratoria is L.m.tibetensis,L.m.manilensis,L.m.migratoria,L.m.migratorioides.
记述了采自摩洛哥土尔蝗属Tuarega1新种,瓦尔扎扎特土尔蝗Tuarega ouarzazatensissp.nov.,模式标本保存于河北大学博物馆。尔瓦扎扎特土尔蝗,新种Tuarega ouarzazatensissp.nov.（图1-6）新种与Tuarega insignis（Lucas,1851）近似,区别特征为：前胸背板沟后区宽大于长,前翅中脉与肘脉不闭合,前翅肘脉有2个分支。正模♀,摩洛哥瓦尔扎扎特东南10km,海拔1100m,2009-03-04,Martin Husemann和Jan Habel博士（NaturalHistory Museum of Luxembourg）采。词源：新种种名出自摩洛哥的瓦尔扎扎特,模式标本产地。
