本文应用生物信息学技术,通过使用三种软件SOPMA、Swiss-model和DNAStar来预测大豆主要过敏原Gly m Bd 30K的抗原表位,结果发现80-85、103-106、217-220、355-360这四段氨基酸残基序列是可能的抗原表位,为后续的蛋白表位实验提供了依据,大大提高了工作效率。
Melamine in combination with cyanuric acid has been considered to be more toxic than either melamine or cyanuric acid alone. The objective of this study was designed to evaluate the combined genotoxicity and cytotoxicity of melamine （M） and cyanuric acid （C） at three mass ratios （1：1, 1：2, 2：1）. MC （1：1）, MC （1：2）, and MC （2：1） were evaluated for their potential genotoxic risk, at gene level by Ames test, and at chromosomal level by micronucleus test. In order to evaluate cytotoxicity in HEK-293 cells, the MTT assay was included. Western blot was also employed to investigate the renal injury molecule-1 （Kim-1） expression in HEK-293 cells exposed to MC. Neither genotoxicity at gene level nor at chromosomal level was observed for MC （1：1）, MC （1：2）, and MC （2：1）. Based on MTT assay, three ratios of MC at 82.5 and 165 μg/mL slightly inhibited viability of HEK-293 cells （P〈0.05）. MC （1：1） at 41.25 and 82.50 IJg/mL could elevate the Kim-1 expression in HEK-293 cells.
LIU XinHUANG Da WeiWU Ke JiaWU Yong NingJIA Xi WuGONG Zhi Yong
