[Objective]The study was carried out for providing good method to detect ratoon stunting disease（RSD）in virus-free seedcane of Saccharum officinarum.[Method]PCR method was used for the detection of RSD in virus-free seedcane of sugarcane.[Result]During the different growth stages of healthy virus-free seedling including proliferation stage,rooting stage,sand culture stage and temporary planting stage,the RSD detection results were negative.PCR detection sensitivity in the present study showed that PCR could detect RSD pathogen in the solution with bacterium concentration 10-3.[Conclusion]PCR method showed a good specificity and higher sensitivity,so it was suitable for the batch detection in the production of healthy virus-free seedlings of sugarcane.
