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国家自然科学基金(30771160)

作品数:9 被引量:43H指数:5
相关作者:曾大力钱前饶玉春庞乾林阮刘青更多>>
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转基因抗虫水稻的生态风险和治理对策被引量:2
2010年
本文综述了转基因抗虫水稻外源抗虫基因的种类和作用机制,探讨了种植转基因抗虫水稻潜在的生态风险,主要包括基因漂移和杂草化、对靶标害虫抗性的影响、对非靶标生物的影响以及对土壤微生态系统的影响,并提出一些相关的治理对策。
饶玉春苏长青钱前曾大力
关键词:转基因抗虫水稻抗虫基因生态风险
Quantitative Trait Loci Mapping of Flag-leaf Ligule Length in Rice and Alignment with ZmLG1 Gene被引量:7
2009年
A doubled haploid (DH) population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid‘CJ06'/‘TNI', was used in this study. Ligule lengths of flag leaf were investigated for quantitative trait loci (QTL) mapping using the DH population. Five QTLs (qLL-2, qLL.4, qLL-6, qLL-IO and qLL-12) controlling the ligule length (LL) were detected on chromosomes 2, 4, 6, 10 and 12, with the variances explained 11.4%, 13.6%, 27.8%, 22.1% and 11.0%, respectively. Using four known genes of ZmGL1, ZmGL2, ZmGL3 and ZmGL4 in maize from the MaizeGDB, their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping. A ZmLG1 homolog gene, OsLG1 encoding a squamosa promoter binding protein, was located between the markers RM255 and RM280, which is just identical to the interval of qLL.4 on the long arm of chromosome 4. The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution.
Dali ZengJiang HuGuojun DongJian LiuLongjun ZengGuangheng ZhangLongbiao GuoYihua ZhouQian Qian
海南国际旅游岛建设给南繁带来的影响被引量:6
2010年
探讨了南繁在我国农业生产中的作用和不可替代性,并在分析海南国际旅游岛建设给南繁工作带来冲击的基础上,建议政府在海南国际旅游岛建设规划中,一并考虑海南南繁——这一中国农业科技硅谷的建设;通过立法对全国各科研单位现有的南繁基地提供必要的法律保护;同时加强国家级的南繁基地建设、增加科研育种工作的财政支持力度,以确保南繁工作的正常开展和"中国农业科技硅谷"的健康发展,进而确保农业科技实力的提升和国家粮食安全体系的巩固。
阮刘青曾大力林海庞乾林
关键词:国际旅游岛南繁
Characterization and Fine Mapping of Non-panicle Mutant (nop) in Rice
2009年
A mutant of panicle differentiation in rice called non-panicle (nop) was discovered in the progeny of a cross between 93-11 and Nipponbare. The mutant exhibits normal plant morphology but has apparently few tillers. The most striking change in nop is that its panicle differentiation is blocked, with masses of fluffy bract nodes generate from the positions where rachis branches normally develop in wild-type plants. Genetic analysis suggests that nop is controlled by a single recessive gene, which is temporarily named Nop(t). Based on its mutant phenotype, Nop(t) represents a key gene controlling the initiation of inflorescence differentiation, By using simple sequence repeat markers and sequence tagged site markers, Nop(t) gene was fine mapped in a 102-kb interval on the long arm of chromosome 6. These results will facilitate the positional cloning and functional studies of the gene.
Wu KunRAO Yu-chunHu JiangZHU Guan-linZHANG Guang-henHu Xin-mingGuo Long-biaoWANG Yong-hongQIAN QianZENG Da-li
Analysis on Rice Production and International Trade in Vietnam
2011年
This paper introduces Vietnam's climate condition,main rice production regions and analyses the expansion of rice planting area,rice cropping system during the last decades.The result from the change of rice production,planting area,yield,and rice trade indicates that the economic reforms in Vietnam from 1986 have contributed to a spectacular rise in rice production and exports.However,there are still problems and opportunities for rice production and export in Vietnam.The paper suggests that Vietnam should make the most use of the advanced international rice cultivars and technology to improve irrigation and water conservancy facilities to benefit rice farmer and consolidate Vietnam to be the major exporter of rice in the world market.
RUAN Liu-qingJIANG Yun-zhuLU Li-xiangLIN HaiPANG Qian-lin
关键词:VIETNAM
A Simple Method for Preparation of Rice Genomic DNA被引量:4
2010年
The extraction of DNA is often the most time consuming and laborious step in high-throughput molecular genetic analysis and marker assisted selection (MAS) programs. A simple method for preparation of rice genomic DNA was developed. A small amount (1~50 mg) of leaf tissue of rice seedling, 500 pL of extraction buffer, and one steel bead were put into a 2-mL microcentrifuge tube. After vigorously mashing for 2 min, 5 μL of supernatant was directly applied to PCR amplification. Otherwise, the supematant was precipitated with two times volume of ethanol to obtain high quality genomic DNA. This method is simple, rapid, low cost, and reliable for PCR analysis. One person can manipulate as many as 96 samples for PCR in 10 min. It is especially suitable for genotyping of large number of samples.
SUN ChuanHE Ying-hongCHEN GangRAO Yu-chunZHANG Guang-hengGAO Zhen-yuLIU JianJu Pei-naHu JiangGuo Long-biaoQIAN QianZENG Da-li
关键词:RICE
水稻基因组DNA简易制备方法被引量:13
2010年
介绍了一种用于PCR的水稻基因组DNA的快速制备方法。该方法只需将少量(1~50mg)样品、适量提取液(500μL)和一粒钢珠放入2mL离心管,在细胞破碎仪中粉碎2min,经离心后可直接取少量(约5μL)上清液作为PCR的模板DNA,也可进一步根据需要实现高质量DNA的提取。该方法具有成本低、操作快速简便等优点,一个人可以在10min内完成96份样品DNA的简易制备,特别适合高通量的分子检测。
孙川陈刚饶玉春张光恒高振宇刘坚鞠培娜胡江郭龙彪钱前曾大力
关键词:DNA提取水稻
越南水稻生产与国际贸易情况分析被引量:7
2011年
概述了越南水稻生产的自然条件和种植区域分布;从越南水稻总产、收获面积、单产及稻米国际贸易情况的变化可以看出,越南自1986年政策改革以来,水稻生产取得了令人瞩目的成就,但仍面临多方面的问题,今后应进一步引进和推广杂交水稻,并增强农田基础设施建设等措施,以提高水稻产量。
阮刘青江云珠吕利湘林海庞乾林
关键词:水稻生产稻米贸易
Genetic analysis of leaffolder resistance in rice被引量:6
2010年
A double haploid (DH) population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid ‘CJ06'/‘TNI', was used to investigate the genetic basis for rice leaffolder resistance. Using a constructed molecular linkage map, five QTLs for rolled leaves were detected on chromosomes 1, 2, 3, 4, and 8. The positive alleles from C J06 on chromosomes 3, 4, and 8 increased the resistance to rice leaffolder, and the alleles from TN1 on chromosomes 1 and 2 also enhanced resistance to leaffolder. The interactions between QTLs were identified and tested, and four conditional interactions were acquired for resistance to rice leaffolder. These loci were located on chromosomes 2, 9, 10, and 11, respectively. QTL pyramiding indicated that the positive alleles affect resistance to leaffolder. The prospective application of this data in rice breeding was also discussed.
Yuchun RaoGuojun DongDali ZengJiang HuLongjun ZengZhengyu GaoGuanghen ZhangLongbiao GuoQian Qian
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