搜索到5378篇“ THUNB“的相关文章
同步辐射μ-XRF用于金银花中砷的吸收分布和转运趋势研究
2025年
金银花因环境因素影响,砷超标问题严重。采用水培法,模拟有机砷与无机砷胁迫,探究金银花的主要酶活性变化,并应用同步辐射X射线荧光光谱(μ-XRF)技术解析砷在金银花中的吸收、分布及转运趋势。使用不同形态不同浓度的砷胁迫金银花植株,测定叶片的生化指标,包括过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD),生化结果显示:在低浓度砷胁迫下,多个生化指标能够积极响应,低浓度无机砷与高浓度有机砷胁迫对POD活性的影响相近,两种形态的砷长时间胁迫,都会使POD显著上升;高浓度有机砷胁迫使CAT急剧下降,表明植物自身的保护机制受损;SOD的变化因砷形态而异,无机砷条件下呈V型波动,有机砷条件下则持续降低,表明植物细胞调节能力受限。μ-XRF分析显示:无机砷主要蓄积于植物的根部表皮层,根与茎共同形成拦截屏障;有机砷主要蓄积于金银花根部维管柱。这为评估重金属污染对药材品质的影响及探索减少药用部位重金属积累策略提供科学依据。
周俊彤张伊彤彭聪男霍清刘美
关键词:金银花抗氧化酶
金银花绿原酸基因的发掘及生物信息学分析
2024年
绿原酸(chlorogenic acid, CGA)是金银花(Lonicera japonica Thunb.)重要的功效成分,具有抗菌、抗病毒、防癌、防衰老等作用。本研究参照17个物种中33个绿原酸基因编码蛋白的氨基酸序列,通过NCBIBLAST扫描金银花蛋白组数据库,结合亲缘关系分析和结构域分析,筛选获得5个调控金银花绿原酸候选基因,均为Phenylalanine amononia-lyase,分别命名为LjPAL1、LjPAL2、LjPAL3、LjPAL4、LjPAL5。理化性质分析和亚细胞定位发现,这5个候选基因编码的氨基酸个数为686~714,理论等电点为5.85~6.26,均为稳定的亲水性蛋白,均定位在细胞质中。本研究为金银花的分子标记辅助育种提供基因资源,为金银花绿原酸合成代谢途径的研究提供科学依据。
李凤梅陈林鑫李建军张梦齐玉亭徐鑫徐小博陈磊山
关键词:绿原酸同源基因生物信息学分析
Anti-melanogenic properties of FBCC-EP850 derived from Carex pumila Thunb
2024年
Objective:To elucidate the anti-melanogenic potential of Carex pumila Thunb.extract(FBCC-EP850).Methods:A collection of 180 plant extracts was tested for inhibition of mushroom tyrosinase activity using an in vitro assay.Among them,FBCC-EP850 exhibited the most promising inhibitory activity.Further analysis was conducted to investigate its mechanisms and therapeutic potential in reducing melanogenesis in B16F10 melanoma cells and zebrafish larvae.Results:FBCC-EP850 inhibited mushroom tyrosinase activity in a dose-dependent manner,with a half-maximal inhibitory concentration of 45.83μg/mL.FBCC-EP850 at concentrations up to 50μg/mL demonstrated minimal cytotoxicity against B16F10 melanoma cells and no adverse effects on zebrafish larvae.Treatment with 50μg/mL of FBCC-EP850 significantly reducedα-melanocyte stimulating hormone-induced melanin production and suppressed cellular tyrosinase activity in B16F10 melanoma cells.Additionally,FBCC-EP850 at 25 and 50μg/mL effectively diminished hyperpigmentation inα-melanocyte stimulating hormone-stimulated zebrafish larvae.Its anti-melanogenic action could be attributed to modulation of the cAMP-CREB-MITF signaling pathway.Conclusions:Carex pumila extract can inhibit melanogenesis by modulating the cAMP-CREB-MITF signaling pathway,which can be used as a promising candidate for treating hyperpigmentation disorders.
Mirissa Hewage Dumindu KavindaJinkuk ParkNayeong KimYung Hyun ChoiGi-Young Kim
关键词:MELANOGENESISTYROSINASE
不同发育时期忍冬花酚酸类成分含量变化
2024年
目的比较不同发育时期忍冬花酚酸类成分含量,评估忍冬花在不同发育时期的药用价值,为确定适宜采收期提供参考。方法高效液相色谱电喷雾串联质谱(HPLC-ESI-MS/MS)测定12种酚酸类成分含量,多元统计分析忍冬花不同发育时期酚酸类成分含量差异。结果忍冬花酚酸类成分总含量高低为二白期>三青期>大白期>米蕾期>银花期>金花期。结论不同发育时期忍冬花酚酸类成分含量差异显著,开放花中原儿茶酸和绿原酸甲酯含量较高,提示开放花具有独特的开发利用价值。
王爱媛范李想张紫瑄姚丹阳张永清
关键词:忍冬花金银花酚酸类成分
金银花茎的浸提液对旋覆花的化感作用
2024年
为了研究金银花茎的浸提液对旋覆花幼苗的化感作用及利用化感作用进行生物防除的可能性,论文采用水提法提取金银花茎的浸提液.试验设置0.02、0.04、0.06、0.08 g/mL四个质量浓度梯度,并以蒸馏水为对照组(CK),通过测定旋覆花幼苗的形态与生理指标,研究金银花茎的浸提液对旋覆花的化感效应.结果表明,旋覆花幼苗在金银花茎浸提液的作用下,除旋覆花叶片中的含丙二醛量有所升高外,旋覆花的苗高、根长、苗的干质量以及叶片中过氧化物酶活性等都受到抑制,且随着浸提液质量浓度的升高,抑制作用增强.当质量浓度为0.08 g/mL时,苗高、根长、苗的干生物量及过氧化物酶活性与对照相比分别降低了11.95%、24.95%、31.25%、30.90%.由此可得,金银花茎的浸提液对旋覆花幼苗生长有明显的化感抑制作用,因此其对一定区域的旋覆花幼苗的防除是可行的.
代磊周修任简在友田静李玉青李勇超
关键词:金银花旋覆花化感作用
Structural Characterization of Chloroplast Genome in Alpinia japonica(Thunb.)Miq.,a Medicinal Plant of the Genus Alpinia
2024年
The analysis of chloroplast gene characteristics in Alpinia japonica(Thunb.)Miq.is of great significance for developing relevant genetic resources.The high-throughput sequencing and bioinformatic research were performed to analyze the chloroplast genome characteristics of A.japonica.The total chloroplast genome length of A.japonica was 161,906 bp,with a typical circular tetrameric structure.And 133 genes were annotated,comprising 87 protein-coding,38 tRNA,and 8 rRNA genes.Furthermore,22 genes contained two copies,and 18 genes owned introns.Repeat sequence analysis showed that it contains 321 simple sequence repeats(SSRs)and 37 long segment repeats.Compared with the chloroplast genomes of eight representative plants in the genus Alpinia,the gene structure,type,and quantity were relatively conservative.Rps12 was the highest variation site in the entire chloroplast gene.A phylogenetic tree showed that the genus Alpinia was the most closely related to the genus Amomum.Meanwhile,A.japonica is the most closely related to Alpinia chinensis belonging to the genus Alpinia.Overall,the chloroplast genome of a new species was reported in the genus Alpinia,and a basis was provided for the utilization of Alpinia plants as a medical resource.
Wentao ShengXi LeiXinjie ChenQuan Kuang
关键词:CHLOROPLAST
基于抗氧化活性筛选旋覆花提取工艺及干燥技术的研究
2024年
目的以单因素和效应面法(Box-Behnken)结合探究旋覆花提取工艺,并评价不同干燥方式对提取物抗氧化能力的影响。方法以麝香草酚含量和浸膏得率为指标,在单因素的基础上,考察料液比、提取时间、提取次数对提取工艺的影响。通过测定提取物对DPPH自由基清除率,比较5种不同干燥方式的影响。结果最佳提取工艺为料液比为1∶30,提取时间为40 min,提取1次;重复工艺所得麝香草酚含量和浸膏得率符合预测值;真空冷冻干燥对DPPH自由基清除率最高。结论所得参数可作为旋覆花提取物的制备工艺参数,并为开发旋覆花抗氧化产品生产时的干燥工艺提供科学依据。
胡晓莲盛芳玲黄学娣唐莉华
关键词:旋覆花效应面法
不同叶面肥对茅苍术种子种苗生长及苍术素含量的影响
2024年
结合十堰市茅苍术[Atractylodes lancea(Thunb.)DC.]种子种苗生产需求,以一年生茅苍术种子种苗为材料,开展不同叶面肥对种苗的生物量和干燥根茎苍术素含量的影响研究。结果表明,不同叶面肥均未能促进一年生茅苍术种苗植株增高,对须根长和须根数无显著影响,减少了地下根茎鲜重和干重,相对提高了干重占比,但显著提高了一年生茅苍术种苗根茎的苍术素含量。此外,尽管叶面肥采用尿素能够提高一年生茅苍术种苗的叶片生长,但叶面肥采用磷酸二氢钾和硝酸钾有利于提高一年生茅苍术种苗的苍术素积累,提高了品质,更有利于大田生产应用。
何亚慧陈盛虎杨永志周明李坤周军兰玉梅贾礼桂司海倩
关键词:叶面肥种苗苍术素
广丰千金薯叶绿体基因组序列特征及密码子使用偏好性分析
2024年
目的 解析广丰千金薯Dioscorea opposite叶绿体基因组信息序列特征和确定其在薯蓣属的系统位置。方法 利用高通量测序技术对广丰千金薯叶片进行核基因组重测序,并利用生物信息学相关工具对组装得到的叶绿体基因组进行注释、序列特征和系统发育分析。结果 广丰千金薯叶绿体基因组长度为153 293 bp,注释到84个CDS基因、31个tRNA基因和8个rRNA基因。广丰千金薯叶绿体基因组中共检测到45个SSR位点,多为单碱基(A/T)重复类型。广丰千金薯叶绿体基因组共鉴定到61个长重复序列,包括16个正向重复、21个回文重复、14个反向重复、10个互补重复。广丰千金薯叶绿体基因组高变区主要分布在LSC和SSC区域,基因间隔区matK-tnaQ-UUG、atpl-rps2、psbl-trnS、rpl32-ndhF、ndhD-ccsA、trn E-UUC-trn T-GGU、trn S-GCU-trnG-UCC、rps2-rpo C2以及基因ycf1变异率最高。广丰千金薯叶绿体基因组的密码子偏性较弱。广丰千金薯叶绿体基因组密码子偏好以A、U结尾。广丰千金薯叶绿体基因组密码子使用偏性很大程度上受自然选择的影响,而受突变压力的影响小。广丰千金薯叶绿体基因组的最优密码子共16个,即UUA、UCU、GAU、GGA、AAU、CAU、GUA、CAA、GAA、CCU、GUU、AUU、CGA、CUU、UUU、AGU。广丰千金薯与野脚板薯Dioscorea polystachya NC_037716聚为一小支。结论 广丰千金薯与野脚板薯NC_037716亲缘关系较近。首次采用生物信息学分析方法对广丰千金薯叶绿体基因组的序列特征及密码子使用偏好性进行了分析,结果可为薯蓣属植物的遗传多样性分析提供理论依据。
尹明华范馨月付悦欣胡慧玲
关键词:叶绿体基因组系统发育分析
Geographic variation in secondary metabolites contents and their relationship with soil mineral elements in Pleuropterus multiflorum Thunb.from different regions
2024年
Background:Pleuropterus multiflorum Thunb.cv.“Heshouwu”(HSW)has been used as a classical material for both medicine and food in China for millennia.Recently,the cultivation region of HSW has shifted from Guangdong to Sichuan,Guizhou,and other regions.The investigation of geographic variation in bioactive metabolite contents and their relationship with soil mineral elements holds academic significance.Objective:This study aimed to investigate the variations in the distribution of active components in HSWacross diverse planting regions and their relationship with soil mineral elements.Methods:A reliable quantitative analysis based on ultrahigh-performance liquid chromatography with triple-quadrupole mass spectrometry(UPLC-QQQ-MS)was developed to assess the levels of 15 bioactivemetabolites in 60 HSWsamples collected from 4 distinct regions.A total of 43 soil mineral elements in corresponding 60 soil samples were quantified by inductively coupled plasma mass spectrometry(ICP-MS).Orthogonal partial least squares-discriminant analysis(OPLS-DA),heatmap analysis,Pearson correlation analysis,and random forest(RF)regression were conducted based on the above quantitative data.Results:The content of stilbene glycosides displayed a wider range of variation compared with emodin and physcion among different regions.Eight compounds were screened as the differential metabolites in HSW samples from various sources using the supervised OPLS-DA analysis.Among these,2 important functional compounds including physcion and 2,3,5,4′-tetrahydroxystilbene-2-O-(6″-O-acetyl)-glucoside(THSG-5)are the most abundant inHSWsamples from Deqing,a geographical indicative production region.Pearson correlation analysis indicated that the impact of soil mineral elements on the levels of stilbene glycosides is greater compared to that on anthraquinones.A negative correlation was observed between the levels of elements Na,Zn,Ba,Ti,and 2,3,5,4′-tetrahydroxysilbene 2-O-glucoside(THSG-1).Conversely,a positive correlation was found between the co
Yaling YangSiman WangRuibin BaiFeng XiongYan JinHanwei LiuZiyiWangChengyuan YangYi YuApu ChowdhuryChuanzhi KangJian YangLanping Guo
关键词:ANTHRAQUINONES

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巩江
作品数:277被引量:1,394H指数:16
供职机构:西藏民族大学
研究主题:药学 药理 化学成分 养生 药理作用
倪士峰
作品数:344被引量:2,033H指数:19
供职机构:西北大学生命科学学院
研究主题:药学 药理 化学成分 药理作用 养生
郑斌
作品数:4被引量:16H指数:3
供职机构:湖北科技学院核技术与化学生物学院
研究主题:白花败酱 THUNB 生理 叶面喷肥 生长生理
张永清
作品数:568被引量:3,368H指数:26
供职机构:山东中医药大学药学院
研究主题:金银花 忍冬 化学成分 丹参 栝楼
王会
作品数:3被引量:5H指数:2
供职机构:襄阳职业技术学院
研究主题:DIOSCOREA THUNB 快繁 组织培养快繁 培养基