目的探讨nm23基因产物和细胞外基质抗原的表达与声门上型喉鳞癌颈淋巴结转移的关系。方法分别应用nm23/NDPK多克隆抗体、抗Ⅳ型胶原纤维(Type IV Collagen,CTⅣ)单克隆抗体、抗层粘连蛋白(Laminin,LN)单克隆抗体、抗纤维连接蛋白(Fibronectin,FN)单克隆抗体以链霉素亲生物素过氧化酶免疫组织化学法观察nm23/NDPK和这三种抗原在72例声门上型喉鳞癌中的表达分布情况及其与颈淋巴结转移的关系。统计学处理采用x2检验。结果声门上型喉鳞癌中nm23/NDPK阴性者发生颈淋巴结转移率高于表达阳性者,二者之间有较强相关性(P<0.01)。CTⅣ、LN、FN表达弱阳性者发生颈淋巴结转移率高于强阳性表达者,也表明其间有较强相关性(P<0.01)。结论nm23/NDPK、CTⅣ、LN、FN表达情况可作为预测声门上型喉鳞癌发生颈淋巴结转移的重要参数之一。
Human Nm23 family consists of eight gene products that have been implicated in cellular differentiation,development and apoptosis,as well as oncogenesis and tumor metastasis.The protein products of Nm23 are nucleoside diphosphate kinases(NDPKs),the key metabolic enzymes that catalyze the synthesis of nucleoside triphosphates(NTP) by transfer of the terminal phosphate between NDP and NTP.Recent investigations are focused on the extraordinary pleiotropy and its mechanism of Nm23/NDPK.In this article,we review the recent progress in studies of the mechanism of Nm23/NDPK as metastasis suppressors,and other bioactivities of NDPK out of phosphate transfer enzyme,as well as the character of NDPK’s quaternary structure and the relation between its structure and function.In addition to these,the potential applications of NDPK as an enhancer of antiviral drugs or Nm23 as a drug target were also described.Findings herein summarized provide new and intriguing suggestions for a more extensive understanding of the biological functions of the star molecule,Nm23/NDPK.
