Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.
Yuhao LiuSonghang LiShiyu LinSirong ShiTaoran TianBowen ZhangTao ZhangYunfeng Lin
Rechargeable sodium ion battery(SIB)has attracted much attention recently.However,the deficiency of high-performance electrode materials limits its commercial development.Exploring new cost-effective,high safe electrode materials and full battery matching technology is an important direction of future research.In this work,a novel watermelon-like multicore-shell Fe(PO_(3))_(2)@C nanocapsule anode material is designed via a facile and eco-friendly process for high performance SIB.Fe(PO_(3))_(2)@C composite anode exhibits remarkable electrochemical performances for SIB,showing high sodium storage capacity(452 mAh·g^(-1) at 0.2 A·g^(-1)),good rate(235 mAh·g^(-1) at 10 A·g^(-1)),stable long-term cycling life(210 mAh·g^(-1) over 2,000 cycles under 5 A·g^(-1)),and superior high-low temperature performance.Furthermore,a new type all iron-based phosphate full battery with high specific capacity is constructed,which can output initial capacity of 309 mAh·g^(-1) and a high energy density of 254,107,and 82 Wh·kg^(-1) at the power density of 186,917,and 1,640 W·kg^(-1) at room temperature.The exceptional performance of multicoreshell Fe(PO_(3))_(2)@C nanocapsule structure can be ascribed to the large specific surface,good structure stability,high conductivity,as well as the multiple layer protection for superior electron/ion transportation.
Lu YueJingyu ZhangMeng KongKai LiWenhui ZhangXiaotian GuoMengmeng XiaoFeng ZhangHuan Pang
Telomerase plays an essential role in many biological processes.DNA methylation regulates the expression of many genes,including telomerase.Here,we propose a deformable satellite nanocapsule fluorescein isothiocyanate(FITC)-hollowbowl mesoporous organicsilica@gold nanoparticles-methyl-CpG-binding protein 2(MECP 2)-silver nanoclusters(FHBMO@AMA),for simultaneous quantitative detection of both cytoplasmic telomerase activity and the degree of DNA methylation.This strategy enabled spatial-based detection in cells.The total cytoplasmic telomerase activity was detected by fluorescence energy resonance transfer(FRET)between FHBMO and gold nanoparticles(Au NPs),while the DNA methylation in the nucleus was detected by enhanced fluorescence of silver nanoclusters(Ag NCs).Furthermore,FHBMO@AMA could intuitively distinguish between the differences in telomerase expression in cells during the DNA synthesis period at the mitotic phase(S/M)of the cell cycle.Interestingly,the ratio of the two detections(telomerase activity/DNA methylation)significantly correlated with the efficacy of anticancer drugs.At the same time,there was no apparent linear relationship between any single detection target and the efficacy of the anticancer drugs.Therefore,based on the relationship between telomerase activity and DNA methylation,our newly developed approach serves as new and feasible method for evaluating the efficacy of anticancer drugs,thereby,extending the technology toolbox for precision in medical and pharmaceutical analysis of drug potency.
The controlled self-assembly of discrete metal-organic nanocapsules(MONCs),and metal-organic frameworks(MOFs)based on the MONCs are achieved.Specifically,the solvothermal reaction of nickel nitrate hexahydrate and C-methylpyrogallol[4]arene in mixed DMF/MeOH solution leads to the unexpected form of discrete nickel-seamed hexameric pyrogallol[4]arene MONCs,and MONC-based three-dimensional(3D)MOF.Notably,the latter MOF is constructed from the aforementioned nickelseamed MONC building blocks and formate linkers in-situ generated from the hydrolysis of DMF solve nt.Interestingly,introducing pyridine and formic acid in the reaction conditions leads to the controlled assemblies of the discrete MONC and MONC-based 3D MOF structures.Moreover,the variabletemperature magnetic susceptibilities of both the abovementioned compounds have been investigated,indicating typical antiferromagnetic interactions between the metal centers.
