目的探讨功能性近红外光谱成像(functional near-infrared spectroscopy,fNIRS)对重性抑郁障碍(Major Depressive Disorder,MDD)患者的N-back表现及相关脑区激活情况。方法去符合美国《精神障碍诊断与统计手册第5版》(diagnostic and statistical manual of mental disorders,5th Edition,DSM-5)诊断标准的抑郁障碍患者26例为研究对象,并选择22名正常成人作为对照。使用17项汉密尔顿抑郁量表(17-item Hamilton depression scale,HAMD-17)评定患者抑郁症状,数字N-back测试评估工作记忆,应用fNIRS测量执行N-back任务时额叶脑区的激活情况。结果①在正确率和反应时间上,负荷水平主效应显著(P<0.05),组别和负荷水平的交互作用显著(P<0.05)。进行简单效应分析发现,在0-back条件下,MDD患者的正确率和反应时间与健康成人不存在显著差异(P>0.05),在1-back和2-back条件下,MDD患者的正确率和反应时间存在显著性差异(P<0.05)。②与健康对照组相比,MDD患者在2-back任务中,背外侧前额叶皮层(通道3、4、5、6、8)和额极皮层(通道47)氧合血红蛋白(Oxyhemoglobin,Oxy-Hb)的激活明显减少(P<0.05)。结论MDD患者fNIRS实验测试所见证据是背外侧前额叶皮层和额极皮层激活减弱。这一初步结果有待于进一步证实。
Objective:To investigate the effect of acupuncture onset time and synaptic structure in two different models of depression.Methods:A total of 150 healthy male adult SPF C57BL/6J mice were divided into five time-point groups:1 h,3 h,6 h,12 h and 24 h.Each time-point group was further divided into three groups:the model,scopolamine and acupuncture groups with 10 rats each.Rats were given forced swimming stimulation(15 min,once)before the intervention.A total of 200 healthy male adult specific pathogen-free(SPF)Sprague-Dawley(SD)rats were divided into 30 normal rats,and 170 modelling ones.A total of 90 rats were successfully modeled,and were randomly divided into three time-point groups:1 h,6 h and 24 h.And each time-point group was further divided into the normal,model,scopolamine,and acupuncture group.Chronic unpredictable mild stress(CUMS)combined with individual feeding was used to establish the depressed rat model.The sucrose preference test(SPT)and open field test(OFT)were used to evaluate the success of the model.There were 10 rats in each group.The acupuncture intervention was performed once for 20 min on"Baihui(GV20)","Yintang(GV29)","Hegu(LI4)",and"Taichong(LR3)"without any other operations for the corresponding animals.Other groups were given corresponding medications.According to different time points,the forced swimming test(FST)was conducted in mice,and the FST and novelty-suppressed feeding test(NSFT)were measured in rats.Monoamine neurotransmitters(NE,DA,DOPAC,5-HT,5-HIAA,HVA)in the prefrontal cortex and hippocampus were detected with high performance liquid chromatography(HPLC).The expression of synapsin I,PSD95,p-mTOR,mTOR,and BDNF in the prefrontal cortex and hippocampus was determined by western blot.Results:At the 1 h time point,compared with the model group,the immobility time was significantly decreased in the acupuncture group(P<0.05).At 3 h,6 h,12 h,and 24 h,compared with the model group,the immobility time was significantly reduced in both scopolamine and acupuncture groups(P<0.05).At the 1
