采用压力驱动的亲和毛细管电泳技术(P-ACE)分别研究了生理酸度条件下(p H 7.4)3种黄酮类化合物龙血素A、龙血素B和剑叶龙血素C与人血清白蛋白(HSA)之间的相互作用。利用蛋白淌度变化和药物浓度的关系,计算得出上述三者与HSA的结合常数Ka分别为0.414×105,0.252×105,1.816×105L/mol。结果表明,P-ACE可作为研究药物与蛋白相互作用的简便可行方法。
HIV-1 gains entry into target cells by sequentially interacting with cellular receptors and co-receptors. Both the receptor and co-receptor are recognized by HIV-1 envelope protein gpl20, which plays a key role in the entry process of HIV-1 into cells. The development of new inhibitors is essential since the viral enzyme reverse transcriptase （RT） is one of the first targets of antiretroviral therapy. It has been reported that a variety of natural plants, such as Artemisia rupestris L., have anti-viral pharmacological activity, and they might be the potential inhibitors of RT or V3 loop of gpl20 against HIV-1. RIQRGPGRAFVT1GK （R15K）, the relatively conserved region of V3 loop, can be used for binding research. In this work, we analyzed the interactions between different extracts from Artemisia rupestris L. and R15K by affinity capillary electrophoresis （ACE）. Moreover, we analyzed the interactions between different extracts from Artemisia rupestris L. and RT by capillary zone electrophoresis （CZE）. Our data showed that the chloroform extract ofArtemisia rupestris L. was active among the different plant extracts, which was consistent with previous studies. Taken together, our study provided a rapid screening method to seek anti-HIV ingredients in natural plants＇ extracts.
